LOCUS       sequence-24765-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-D77
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..273
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(330..348)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      344..391
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(757..762,763..779,780..785))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 caggttcntc tgatattttg tttactttaa gaaggagata tacatatgca gatcttcgtc
       61 aagacgttaa ccggtaaaac cataactcta gaagttgaac catccgatac catcgaaaac
      121 gttaaggcta aaattcaaga caaggaaggc attccacctg atcaacaaag attgatcttt
      181 gccggtaagc agctcgagga cggtagaacg ctgtctgatt acaacattca gaaggagtcg
      241 accttacatc ttgtcttaag actaagaggt ggtgactgag gatccggctg ctaacaaagc
      301 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
      361 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc
      421 acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga
      481 gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa
      541 attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat
      601 ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacacnct
      661 ccagggtgac ggtgccgagg atgacgatga gccnnttgtt agatttcnta cacngctgcc
      721 tgactgcgtt agccatttaa ctgtgataac taccgcatta aagcttatcg atgataagct
      781 gtcaacatga gattcttgaa acgaagggct cgtgatcgct atttataggt angnctgata
      841 tatggttcta nactcagtg
//
