LOCUS       sequence-24766-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-D77
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..270)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             278..300
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(331..350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(332..350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(434..453)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcannctcct ttcgggcttt gttagcagcc ggatcctcag tcaccacctc ttagtcttaa
       61 gacaagatgt aaggtcgact ccttctgaat gttgtaatca gacagcgttc taccgtcctc
      121 gagctgctta ccggcaaaga tcaatctttg ttgatcaggt ggaatgcctt ccttgtcttg
      181 aattttagcc ttaacgtttt cgatggtatc ggatggttca acttctagag ttatggtttt
      241 accggttaac gtcttgacga agatctgcat atgtatatct ccttcttaaa gttaaacaaa
      301 attatttcta gagggaaacc gttgtggtct ccctatagtg agtcgtatta atttcgcggg
      361 atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      421 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      481 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      541 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      601 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
      661 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
      721 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
      781 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga t
//
