LOCUS       sequence-247663-        2347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PatpI (NEP)
FEATURES             Location/Qualifiers
     source          1..2347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(553..570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(663..1251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(743..762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1425..2219)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2220..2324)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcaggag tccttgatta ataataacat aataagatat
      421 acttgagacc ggtaccctct gggcctcatg ggccttcctt tcactgcccg ctttccagtc
      481 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc
      541 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt
      601 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1261 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1321 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1381 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttattagaa aaattcatcc
     1441 agcagacgat aaaacgcaat acgctggcta tccggtgccg caatgccata cagcaccaga
     1501 aaacgatccg cccattcgcc gcccagttct tccgcaatat cacgggtggc cagcgcaata
     1561 tcctgataac gatccgccac gcccagacgg ccgcaatcaa taaagccgct aaaacggcca
     1621 ttttccacca taatgttcgg caggcacgca tcaccatggg tcaccaccag atcttcgcca
     1681 tccggcatgc tcgctttcag acgcgcaaac agctctgccg gtgccaggcc ctgatgttct
     1741 tcatccagat catcctgatc caccaggccc gcttccatac gggtacgcgc acgttcaata
     1801 cgatgtttcg cctgatgatc aaacggacag gtcgccgggt ccagggtatg cagacgacgc
     1861 atggcatccg ccataatgct cactttttct gccggcgcca gatggctaga cagcagatcc
     1921 tgacccggca cttcgcccag cagcagccaa tcacggcccg cttcggtcac cacatccagc
     1981 accgccgcac acggaacacc ggtggtggcc agccagctca gacgcgccgc ttcatcctgc
     2041 agctcgttca gcgcaccgct cagatcggtt ttcacaaaca gcaccggacg accctgcgcg
     2101 ctcagacgaa acaccgccgc atcagagcag ccaatggtct gctgcgccca atcatagcca
     2161 aacagacgtt ccacccacgc tgccgggcta cccgcatgca ggccatcctg ttcaatcata
     2221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2341 gtgccac
//
