LOCUS       sequence-24767-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-K48C
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..272
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGCQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(326..344)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      340..387
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cngttctctg aatattttgt ttactttaag aaggagatat acatatgcag atcttcgtca
       61 agacgttaac cggtaaaacc ataactctag aagttgaacc atccgatacc atcgaaaacg
      121 ttaaggctaa aattcaagac aaggaaggca ttccacctga tcaacaaaga ttgatctttg
      181 ccggttgcca gctcgaggac ggtagaacgc tgtctgatta caacattcag aaggagtcga
      241 ccttacatct tgtcttaaga ctaagaggtg gttgaggatc cggctgctaa caaagcccga
      301 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      361 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag
      421 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
      481 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
      541 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
      601 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cnncnnccnn
      661 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcaacacng tgcctgaact
      721 gcgttagcaa tttaactgtg ataactaccg cattaagctt atcgatgata ngctgtcaac
      781 atgagantct gaaacgaagg nctcgtgaac gctattttat aggtaatgnc tgatatatgg
      841 ttctaactca gg
//
