LOCUS       sequence-247671-        2456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PclpP (PEP)
FEATURES             Location/Qualifiers
     source          1..2456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(662..679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(772..1360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(852..871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1534..2328)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2329..2433)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccagg tctcaggaga aagagctcgg actttattca
      421 ttctgtcttt ctttatgaat ttttataatc tatggataaa ataaatacga taaaaaccaa
      481 tatgaatatt ataaagacaa taaaaaaaat tgttactact tgagaccatt aattaatgga
      541 gcacaagact ggcctcatgg gccttccttt cactgcccgc tttccagtcg ggaaacctgt
      601 cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt
      661 cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc
      721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1201 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1261 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1321 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1381 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1441 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1501 aagtatatat gagtaaactt ggtctgacag ttattagaaa aattcatcca gcagacgata
     1561 aaacgcaata cgctggctat ccggtgccgc aatgccatac agcaccagaa aacgatccgc
     1621 ccattcgccg cccagttctt ccgcaatatc acgggtggcc agcgcaatat cctgataacg
     1681 atccgccacg cccagacggc cgcaatcaat aaagccgcta aaacggccat tttccaccat
     1741 aatgttcggc aggcacgcat caccatgggt caccaccaga tcttcgccat ccggcatgct
     1801 cgctttcaga cgcgcaaaca gctctgccgg tgccaggccc tgatgttctt catccagatc
     1861 atcctgatcc accaggcccg cttccatacg ggtacgcgca cgttcaatac gatgtttcgc
     1921 ctgatgatca aacggacagg tcgccgggtc cagggtatgc agacgacgca tggcatccgc
     1981 cataatgctc actttttctg ccggcgccag atggctagac agcagatcct gacccggcac
     2041 ttcgcccagc agcagccaat cacggcccgc ttcggtcacc acatccagca ccgccgcaca
     2101 cggaacaccg gtggtggcca gccagctcag acgcgccgct tcatcctgca gctcgttcag
     2161 cgcaccgctc agatcggttt tcacaaacag caccggacga ccctgcgcgc tcagacgaaa
     2221 caccgccgca tcagagcagc caatggtctg ctgcgcccaa tcatagccaa acagacgttc
     2281 cacccacgct gccgggctac ccgcatgcag gccatcctgt tcaatcatac tcttcctttt
     2341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
