LOCUS       sequence-247673-        2368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-IEE
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(574..591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(684..1272)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(764..783)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1446..2240)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2241..2345)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcaggag taggatcgtt tatttacaac ggaatggtat
      421 acaaagtcaa cagatctcaa tacttgagac cggtaccctc tgggcctcat gggccttcct
      481 ttcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac atggtcatag
      541 ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc tgcgctcggt
      601 cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt
      661 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      721 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      781 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      841 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      901 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      961 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1021 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1081 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1141 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1201 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1261 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1321 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1381 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1441 agttattaga aaaattcatc cagcagacga taaaacgcaa tacgctggct atccggtgcc
     1501 gcaatgccat acagcaccag aaaacgatcc gcccattcgc cgcccagttc ttccgcaata
     1561 tcacgggtgg ccagcgcaat atcctgataa cgatccgcca cgcccagacg gccgcaatca
     1621 ataaagccgc taaaacggcc attttccacc ataatgttcg gcaggcacgc atcaccatgg
     1681 gtcaccacca gatcttcgcc atccggcatg ctcgctttca gacgcgcaaa cagctctgcc
     1741 ggtgccaggc cctgatgttc ttcatccaga tcatcctgat ccaccaggcc cgcttccata
     1801 cgggtacgcg cacgttcaat acgatgtttc gcctgatgat caaacggaca ggtcgccggg
     1861 tccagggtat gcagacgacg catggcatcc gccataatgc tcactttttc tgccggcgcc
     1921 agatggctag acagcagatc ctgacccggc acttcgccca gcagcagcca atcacggccc
     1981 gcttcggtca ccacatccag caccgccgca cacggaacac cggtggtggc cagccagctc
     2041 agacgcgccg cttcatcctg cagctcgttc agcgcaccgc tcagatcggt tttcacaaac
     2101 agcaccggac gaccctgcgc gctcagacga aacaccgccg catcagagca gccaatggtc
     2161 tgctgcgccc aatcatagcc aaacagacgt tccacccacg ctgccgggct acccgcatgc
     2221 aggccatcct gttcaatcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2281 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2341 ccgcgcacat ttccccgaaa agtgccac
//
