LOCUS       sequence-247676-        2370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PpsbA
FEATURES             Location/Qualifiers
     source          1..2370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(576..593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(686..1274)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(766..785)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1448..2242)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2243..2347)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcaggag gatctacata caccttggtt gacacgagta
      421 tataagtcat gttatactgt tgtacttgag accggtaccc tctgggcctc atgggccttc
      481 ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat
      541 agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg
      601 gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc
      661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1441 acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg ctatccggtg
     1501 ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt tcttccgcaa
     1561 tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga cggccgcaat
     1621 caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac gcatcaccat
     1681 gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca aacagctctg
     1741 ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg cccgcttcca
     1801 tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga caggtcgccg
     1861 ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt tctgccggcg
     1921 ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc caatcacggc
     1981 ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg gccagccagc
     2041 tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa
     2101 acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag cagccaatgg
     2161 tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg ctacccgcat
     2221 gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg
     2281 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2341 ttccgcgcac atttccccga aaagtgccac
//
