LOCUS       sequence-247679-       13930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13930)
  AUTHORS   Dobrowolski C, Valadkhan S, Graham AC, Shukla M, Ciuffi A, Telenti 
            A, Karn J
  TITLE     Entry of Polarized Effector Cells into Quiescence Forces HIV 
            Latency.
  JOURNAL   MBio. 2019 Mar 26;10(2). pii: mBio.00337-19. doi: 
            10.1128/mBio.00337-19.
  PUBMED    30914509
REFERENCE   2  (bases 1 to 13930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-03-26"; volume: "10(2). pii: mBio.00337-19. doi"; pages:
            " 10.1128/mBio.00337-19"
COMMENT     Sequence Label: pHR-H13LTat-CD8a/d2eGFP-IRES-Nef
FEATURES             Location/Qualifiers
     source          1..13930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3174..3407
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3592..3636
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3930..3956
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      4208..4217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4892..4894,4895..4897,4898..5608,5609..5614,
                     5615..5737)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(4937..4958)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5198..5217)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5545..5566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      5747..5756
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(5765..6336,6337..6339,6340..6348)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5932..5949)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6159..6178
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             6638..7271
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8750..8768)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8751..8768)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9058..9077)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9291..9310)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9410..9432
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9470..9488)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9556..9660
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9661..9729,9730..10521)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9879..9898)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10692..11280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11181..11200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11434..11451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11521..11541)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11526..11855
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11706..11841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12989..13054
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13184..13204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13629..13763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13666..13685)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13720..13739
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc gaattctgca acaactgctg tttatccatt tcagaattgg gtgtcgtcga
     1201 tcgacatagc agaataggcg ttactcgaca gaggagagca agaaatggag ccagtagatc
     1261 ctagactaga gccctggaag ttaccaggaa gtcagcctaa aactgcttgt accaattgct
     1321 attgtaaaaa gtgttgcttt cattgccaag tttgtttcat gacaaaagcc ttaggcatct
     1381 cctatggcag gaagaagcgg agacagcgac gaagagctca tcagaacagt cagactcatc
     1441 aagcttctct atcaaagcag taagtagtac atgtaatgca acctataata gtagcaatag
     1501 tagcattagt agtagcaata ataatagcaa tagttgtgtg gtccatagta atcatagaat
     1561 ataggaaaat attaagacaa agaaaaatag acaggttaat tgatagacta atagaaagag
     1621 cagaagacag tggcaatgag agtgaaggag aagtatcagc acttgtggag atgggggtgg
     1681 aaatggggca ccatgctcct tgggatattg atgatctgta gtgctacaga aaaattgtgg
     1741 gtcacagtct attatggggt acctgtgtgg aaggaagcaa ccaccactct attttgtgca
     1801 tcagatgcta aagcatatga tacagaggta cataatgttt gggccacaca tgcctgtgta
     1861 cccacagacc ccaacccaca agaagtagta ttggtaaatg tgacagaaaa ttttaacatg
     1921 tggaaaaatg acatggtaga acagatgcat gaggatataa tcagtttatg ggatcaaagc
     1981 ctaaagccat gtgtaaaatt aaccccactc tgtgttagtt taaagtgcac tgatttgaag
     2041 aatgatacta ataccaatag tagtagcggg agaatgataa tggagaaagg agagataaaa
     2101 aactgctctt tcaatatcag cacaagcata agagataagg tgcagaaaga atatgcattc
     2161 ttttataaac ttgatatagt accaatagat aataccagct ataggttgat aagttgtaac
     2221 acctcagtca ttacacaggc ctgtccaaag gtatcctttg agccaattcc catacattat
     2281 tgtgccccgg ctggttttgc gattctaaaa tgtaataata agacgttcaa tggaacagga
     2341 ccatgtacaa atgtcagcac agtacaatgt acacatggaa tcaggccagt agtatcaact
     2401 caactgctgt taaatggcag tctagcagaa gaagatgtag taattagatc tgccaatttc
     2461 acagacaatg ctaaaaccat aatagtacag ctgaacacat ctgtagaaat taattgtaca
     2521 agacccaaca acaatacaag aaaaagtatc cgtatccaga ggggaccagg gagagcattt
     2581 gttacaatag gaaaaatagg aaatatgaga caagcacatt gtaacattag tagagcaaaa
     2641 tggaatgcca ctttaaaaca gatagctagc aaattaagag aacaatttgg aaataataaa
     2701 acaataatct ttaagcaatc ctcaggaggg gacccagaaa ttgtaacgca cagttttaat
     2761 tgtggagggg aatttttcta ctgtaattca acacaactgt ttaatagtac ttggtttaat
     2821 agtacttgga gtactgaagg gtcaaataac actgaaggaa gtgacacaat cacactccca
     2881 tgcagaataa aacaatttat aaacatgtgg caggaagtag gaaaagcaat gtatgcccct
     2941 cccatcagtg gacaaattag atgttcatca aatattactg ggctgctatt aacaagagat
     3001 ggtggtaata acaacaatgg gtccgagatc ttcagacctg gaggaggcga tatgagggac
     3061 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     3121 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     3181 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     3241 acggtacagg ccagacaatt attgtctgat atagtgcagc agcagaacaa tttgctgagg
     3301 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa acagctccag
     3361 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     3421 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     3481 tctctggaac agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat
     3541 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3601 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3661 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3721 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3781 cagacccacc tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3841 ggagagagag acagagacag atccattcga ttagtgaacg gatccttagc acttatctgg
     3901 gacgatctgc ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt
     3961 gtaacgagga ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg
     4021 aatctcctac agtattggag tcaggaacta aagaatagtg ctgttaactt gctcaatgcc
     4081 acagccatag cagtagctga ggggacagat agggttatag aagcattaca agcagcttat
     4141 agagctattc gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa
     4201 cacgcgtgcc accatggcct caccgttgac ccgctttctg tcgctgaacc tgctgctgct
     4261 gggtgagtcg attatcctgg ggagtggaga agctaagcca caggcacccg aactccgaat
     4321 ctttccaaag aaaatggacg ccgaacttgg tcagaaggtg gacctggtat gtgaagtgtt
     4381 ggggtccgtt tcgcaaggat gctcttggct cttccagaac tccagctcca aactccccca
     4441 gcccaccttc gttgtctaca tggcttcatc ccacaacaag ataacgtggg acgagaagct
     4501 gaattcgtcg aaactgtttt ctgccatgag ggacacgaat aataagtacg ttctcaccct
     4561 gaacaagttc agcaaggaaa acgaaggcta ctatttctgc tcagtcatca gcaactcggt
     4621 gatgtacttc agttctgtcg tgccagtcct tcagaaagtg aactctacta ctaccaagcc
     4681 agtgctgcga actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga
     4741 agattgtcgg ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta
     4801 catctgggca cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct
     4861 catctgctac cactccggag gcggaggccc catggtgagc aagggcgagg agctgttcac
     4921 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4981 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5041 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5101 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5161 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5221 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5281 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5341 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5401 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5461 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5521 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5581 cactctcggc atggacgagc tgtacaagaa gcttagccat ggcttcccgc cggaggtgga
     5641 ggagcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca
     5701 ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagctc gacgccgcca ccatggaggt
     5761 tacccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5821 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5881 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5941 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     6001 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     6061 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     6121 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     6181 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6241 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     6301 cggggacgtg gttttccttt gaaaaacacg atgataatgg ccacaaccat gggtggcaag
     6361 tggtcaaaaa gtagtgtgat tggatggcct gctgtaaggg aaagaatgag acgagctgag
     6421 ccagcagcag atggggtggg agcagtatct cgagacctag aaaaacatgg agcaatcaca
     6481 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     6541 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     6601 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6661 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     6721 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     6781 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     6841 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     6901 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga gtacttcaag
     6961 aactgctgac atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
     7021 ggcctgggcg ggactgggga gtggcgagcc ctcagatgct gcatataagc agctgctttt
     7081 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7261 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7321 atgaatatca gagagtgaga ggccttgaca ttataataga tttagcagga attgaactag
     7381 gagtggagca cacaggcaaa gctgcagaag tacttggaag aagccaccag agatactcac
     7441 gattctgcac atacctggct aatcccagat cctaaggatt acattaagtt tactaacatt
     7501 tatataatga tttatagttt aaagtataaa cttatctaat ttactattct gacagatatt
     7561 aattaatcct caaatatcat aagagatgat tactattatc cccatttaac acaagaggaa
     7621 actgagaggg aaagatgttg aagtaatttt cccacaatta cagcatccgt tagttacgac
     7681 tctatgatct tctgacacaa attccattta ctcctcaccc tatgactcag tcgaatatat
     7741 caaagttatg gacattatgc taagtaacaa attacccttt tatatagtaa atactgagta
     7801 gattgagaga agaaattgtt tgcaaacctg aatagcttca agaagaagag aagtgaggat
     7861 aagaataaca gttgtcattt aacaagtttt aacaagtaac ttggttagaa agggattcaa
     7921 atgcataaag caagggataa atttttctgg caacaagact atacaatata accttaaata
     7981 tgacttcaaa taattgttgg aacttgataa aactaattaa atattattga agattatcaa
     8041 tattataaat gtaatttact tttaaaaagg gaacatagaa atgtgtatca ttagagtaga
     8101 aaacaatcct tattatcaca atttgtcaaa acaagtttgt tattaacaca agtagaatac
     8161 tgcattcaat taagttgact gcagattttg tgttttgtta aaattagaaa gagataacaa
     8221 caatttgaat tattgaaagt aacatgtaaa tagttctaca tacgttcttt tgacatcttg
     8281 ttcaatcatt gatcgaagtt ctttatcttg gaagaatttg ttccaaagac tctgaaataa
     8341 ggaaaacaat ctattatata gtctcacacc tttgttttac ttttagtgat ttcaatttaa
     8401 taatgtaaat ggttaaaatt tattcttctc tgagatcatt tcacattgca gatagaaaac
     8461 ctgagactgg ggtaattttt attaaaatct aatttaatct cagaaacaca tctttattct
     8521 aacatcaatt tttccagttt gatattatca tataaagtca gccttcctca tctgcaggtt
     8581 ccacaacaaa aatccaacca actgtggatc aaaaatattg ggaaaaaatt aaaaatagca
     8641 atacaacaat aaaaaaatac aaatcagaaa aacagcacag tataacaact ttatttagca
     8701 tttacaatct attaggtatt ataagtaatc tagaattaat tccgtgtatt ctatagtgtc
     8761 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     8821 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     8881 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     8941 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     9001 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     9061 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     9121 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     9181 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     9241 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     9301 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     9361 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     9421 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     9481 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9661 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9721 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9781 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9841 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9901 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9961 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
    10021 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
    10081 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10141 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    10201 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    10261 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    10321 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    10381 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10441 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10501 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10561 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10801 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    11041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    11101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    11161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    11221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    11281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    11341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    11401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    11461 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
    11521 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    11581 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    11641 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
    11701 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    11761 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    11821 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
    11881 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
    11941 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
    12001 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
    12061 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
    12121 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
    12181 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
    12241 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
    12301 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
    12361 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
    12421 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
    12481 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
    12541 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
    12601 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
    12661 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
    12721 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
    12781 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
    12841 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
    12901 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
    12961 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
    13021 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
    13081 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
    13141 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
    13201 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
    13261 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
    13321 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
    13381 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
    13441 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
    13501 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
    13561 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
    13621 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    13681 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    13741 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    13801 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    13861 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    13921 acttagtagt
//
