LOCUS       sequence-24768-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-K48C
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..265)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGCQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             273..295
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(326..345)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(327..345)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(429..448)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atnncttcct tngggcttgt tagcagccgg atcctcaacc acctcttagt cttaagacaa
       61 gatgtaaggt cgactccttc tgaatgttgt aatcagacag cgttctaccg tcctcgagct
      121 ggcaaccggc aaagatcaat ctttgttgat caggtggaat gccttccttg tcttgaattt
      181 tagccttaac gttttcgatg gtatcggatg gttcaacttc tagagttatg gttttaccgg
      241 ttaacgtctt gacgaagatc tgcatatgta tatctccttc ttaaagttaa acaaaattat
      301 ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg tattaatttc gcgggatcga
      361 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      421 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
      481 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
      541 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
      601 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag
      661 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
      721 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
      781 tttcggcnag aacgcnttcg ctgnaaccga cgata
//
