LOCUS       sequence-247683-        2376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PpsbB
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(582..599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(692..1280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(772..791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1454..2248)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2249..2353)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcaggag gaaaatggga tcaactcccc cattgcgtat
      421 tggtacttat cgagtataga ataaatctta cttgagaccg gtaccctctg ggcctcatgg
      481 gccttccttt cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat
      541 ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg
      601 cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca
      661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1441 ggtctgacag ttattagaaa aattcatcca gcagacgata aaacgcaata cgctggctat
     1501 ccggtgccgc aatgccatac agcaccagaa aacgatccgc ccattcgccg cccagttctt
     1561 ccgcaatatc acgggtggcc agcgcaatat cctgataacg atccgccacg cccagacggc
     1621 cgcaatcaat aaagccgcta aaacggccat tttccaccat aatgttcggc aggcacgcat
     1681 caccatgggt caccaccaga tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca
     1741 gctctgccgg tgccaggccc tgatgttctt catccagatc atcctgatcc accaggcccg
     1801 cttccatacg ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca aacggacagg
     1861 tcgccgggtc cagggtatgc agacgacgca tggcatccgc cataatgctc actttttctg
     1921 ccggcgccag atggctagac agcagatcct gacccggcac ttcgcccagc agcagccaat
     1981 cacggcccgc ttcggtcacc acatccagca ccgccgcaca cggaacaccg gtggtggcca
     2041 gccagctcag acgcgccgct tcatcctgca gctcgttcag cgcaccgctc agatcggttt
     2101 tcacaaacag caccggacga ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc
     2161 caatggtctg ctgcgcccaa tcatagccaa acagacgttc cacccacgct gccgggctac
     2221 ccgcatgcag gccatcctgt tcaatcatac tcttcctttt tcaatattat tgaagcattt
     2281 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2341 taggggttcc gcgcacattt ccccgaaaag tgccac
//
