LOCUS       sequence-247689-        2440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PpsbD
FEATURES             Location/Qualifiers
     source          1..2440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(646..663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(756..1344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(836..855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1518..2312)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2313..2417)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caggagcgaa aggttagaaa tcaacaaaag
      421 aaaaagtaag tggacctgac ctattgaatc atgactatat ccgctattct gatattaaaa
      481 ttcgatagag atgaaattgg agctacttga gaccggtacc tggagcacaa gactggcctc
      541 atgggccttc ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      601 acatggtcat agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc
      661 gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag
      721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1501 acttggtctg acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg
     1561 ctatccggtg ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt
     1621 tcttccgcaa tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga
     1681 cggccgcaat caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac
     1741 gcatcaccat gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca
     1801 aacagctctg ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg
     1861 cccgcttcca tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga
     1921 caggtcgccg ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt
     1981 tctgccggcg ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc
     2041 caatcacggc ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg
     2101 gccagccagc tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg
     2161 gttttcacaa acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag
     2221 cagccaatgg tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg
     2281 ctacccgcat gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc
     2341 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2401 caaatagggg ttccgcgcac atttccccga aaagtgccac
//
