LOCUS       sequence-24769-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-K63R
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..272
                     /codon_start=1
                     /product="human ubiquitin variant in which Lys-63 has been 
                     mutated to arginine"
                     /label=ubiquitin-K63R
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     CDS             45..272
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     primer_bind     complement(326..344)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      340..387
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(753..758,759..775,776..781))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ggnttctctg aatattttgt ttactttaag aaggagatat acatatgcag atcttcgtca
       61 agacgttaac cggtaaaacc ataactctag aagttgaacc atccgatacc atcgaaaacg
      121 ttaaggctaa aattcaagac aaggaaggca ttccacctga tcaacaaaga ttgatctttg
      181 ccggtaagca gctcgaggac ggtagaacgc tgtctgatta caacattcag cgggagtcga
      241 ccttacatct tgtcttaaga ctaagaggtg gttgaggatc cggctgctaa caaagcccga
      301 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      361 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag
      421 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
      481 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
      541 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
      601 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
      661 ggtgacggtg ccgaggannc aaatgancgc attgtnagat ttcatacacg gtgcctgact
      721 gcgttagcaa tttaactgtg ataanctacc gcattaaagc ttatcgatga taagctgtca
      781 acatgagatt ctngagacga aggcctcgtg atnncctatt
//
