LOCUS       sequence-24770-e         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET3a-Ub-K63R
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..263)
                     /codon_start=1
                     /product="human ubiquitin variant in which Lys-63 has been 
                     mutated to arginine"
                     /label=ubiquitin-K63R
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     CDS             complement(36..263)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     RBS             271..293
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(324..343)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(325..343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(427..446)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcatactctt cgggcttgta gcagccggat cctcaaccac ctcttagtct taagacaaga
       61 tgtaaggtcg actcccgctg aatgttgtaa tcagacagcg ttctaccgtc ctcgagctgc
      121 ttaccggcaa agatcaatct ttgttgatca ggtggaatgc cttccttgtc ttgaatttta
      181 gccttaacgt tttcgatggt atcggatggt tcaacttcta gagttatggt tttaccggtt
      241 aacgtcttga cgaagatctg catatgtata tctccttctt aaagttaaac aaaattattt
      301 ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga
      361 tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
      421 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
      481 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
      541 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
      601 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag
      661 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
      721 tgactgtctt ctttatcatg caactcgtan gacaggtgcc cggcagcgct ctgggtcatt
      781 ttcngcgaga a
//
