LOCUS       sequence-247714-       15957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15957)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     74..93
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        784..893
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             928..951
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9887..9896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10591..10620
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10621..10644
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10734..10754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11152..11163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11388..11522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11425..11444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11479..11498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11551..11716
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      11900..12354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11987..12006)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12196..12217
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12380..12484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12485..12553,12554..13345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12703..12722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13516..14104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14005..14024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14258..14275
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  14409..14633
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(14700..15233)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(15422..15427,15428..15444,15445..15450))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
       61 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccggtaccg
      121 catgcggatc tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaat
      781 acggaccttt aattcaaccc aacacaatat attatagtta aataagaatt attatcaaat
      841 catttgtata ttaattaaaa tactatactg taaattacat tttatttaca atcactcgac
      901 ggatccttaa gaaggagata tactatggac tacaaagacg atgacgacaa gatggcgacc
      961 ctggaaaagc tgatgaaggc cttcgagtcc ctcaagtcct tccaacagca gcaacagcaa
     1021 caacagcagc aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag
     1081 cagcaacagc aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa
     1141 cagcaacaac agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa
     1201 caacagcagc aacagcaaca acaacagcag caacagcaac aacagcagca acagcaaccg
     1261 ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc agccgccgcc gcaggcacag
     1321 ccgctgctgc ctcagccgca gccgcccccg ccgccgcccc cgccgccacc cggcccggct
     1381 gtggctgagg agccgctgca ccgaccaaag aaagaacttt cagctaccaa gaaagaccgt
     1441 gtgaatcatt gtctgacaat atgtgaaaac atagtggcac agtctgtcag aaattctcca
     1501 gaatttcaga aacttctggg catcgctatg gaactttttc tgctgtgcag tgatgacgca
     1561 gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca aagttatcaa agctttgatg
     1621 gattctaatc ttccaaggtt acagctcgag ctctataagg aaattaaaaa gaatggtgcc
     1681 cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc tggctcacct ggttcggcct
     1741 cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc tgactcgaac aagcaagaga
     1801 cccgaagaat cagtccagga gaccttggct gcagctgttc ccaaaattat ggcttctttt
     1861 ggcaattttg caaatgacaa tgaaattaag gttttgttaa aggccttcat agcgaacctg
     1921 aagtcaagct cccccaccat tcggcggaca gcggctggat cagcagtgag catctgccag
     1981 cactcaagaa ggacacaata tttctatagt tggctactaa atgtgctctt aggcttactc
     2041 gttcctgtcg aggatgaaca ctccactctg ctgattcttg gcgtgctgct caccctgagg
     2101 tatttggtgc ccttgctgca gcagcaggtc aaggacacaa gcctgaaagg cagcttcgga
     2161 gtgacaagga aagaaatgga agtctctcct tctgcagagc agcttgtcca ggtttatgaa
     2221 ctgacgttac atcatacaca gcaccaagac cacaatgttg tgaccggagc cctggagctg
     2281 ttgcagcagc tcttcagaac gcctccaccc gagcttctgc aaaccctgac cgcagtcggg
     2341 ggcattgggc agctcaccgc tgctaaggag gagtctggtg gccgaagccg tagtgggagt
     2401 attgtggaac ttatagctgg agggggttcc tcatgcagcc ctgtcctttc aagaaaacaa
     2461 aaaggcaaag tgctcttagg agaagaagaa gccttggagg atgactctga atcgagatcg
     2521 gatgtcagca gctctgcctt aacagcctca gtgaaggatg agatcagtgg agagctggct
     2581 gcttcttcag gggtttccac tccagggtca gcaggtcatg acatcatcac agaacagcca
     2641 cggtcacagc acacactgca ggcggactca gtggatctgg ccagctgtga cttgacaagc
     2701 tctgccactg atggggatga ggaggatatc ttgagccaca gctccagcca ggtcagcgcc
     2761 gtcccatctg accctgccat ggacctgaat gatgggaccc aggcctcgtc gcccatcagc
     2821 gacagctccc agaccaccac cgaagggcct gattcagctg ttaccccttc agacagttct
     2881 gaaattgtgt tagacggtac cgacaaccag tatttgggcc tgcagattgg acagccccag
     2941 gatgaagatg aggaagccac aggtattctt cctgatgaag cctcggaggc cttcaggaac
     3001 tcttccatgg cccttcaaca ggcacattta ttgaaaaaca tgagtcactg caggcagcct
     3061 tctgacagca gtgttgataa atttgtgttg agagatgaag ctactgaacc gggtgatcaa
     3121 gaaaacaagc cttgccgcat caaaggtgac attggacagt ccactgatga tgactctgca
     3181 cctcttgtcc attgtgtccg ccttttatct gcttcgtttt tgctaacagg gggaaaaaat
     3241 gtgctggttc cggacaggga tgtgagggtc agcgtgaagg ccctggccct cagctgtgtg
     3301 ggagcagctg tggccctcca cccggaatct ttcttcagca aactctataa agttcctctt
     3361 gacaccacgg aataccctga ggaacagtat gtctcagaca tcttgaacta catcgatcat
     3421 ggagacccac aggttcgagg agccactgcc attctctgtg ggaccctcat ctgctccatc
     3481 ctcagcaggt cccgcttcca cgtgggagat tggatgggca ccattagaac cctcacagga
     3541 aatacatttt ctttggcgga ttgcattcct ttgctgcgga aaacactgaa ggatgagtct
     3601 tctgttactt gcaagttagc ttgtacagct gtgaggaact gtgtcatgag tctctgcagc
     3661 agcagctaca gtgagttagg actgcagctg atcatcgatg tgctgactct gaggaacagt
     3721 tcctattggc tggtgaggac agagcttctg gaaacccttg cagagattga cttcaggctg
     3781 gtgagctttt tggaggcaaa agcagaaaac ttacacagag gggctcatca ttatacaggg
     3841 cttttaaaac tgcaagaacg agtgctcaat aatgttgtca tccatttgct tggagatgaa
     3901 gaccccaggg tgcgacatgt tgccgcagca tcactaatta ggcttgtccc aaagctgttt
     3961 tataaatgtg accaaggaca agctgatcca gtagtggccg tggcaagaga tcaaagcagt
     4021 gtttacctga aacttctcat gcatgagacg cagcctccat ctcatttctc cgtcagcaca
     4081 ataaccagaa tatatagagg ctataaccta ctaccaagca taacagacgt cactatggaa
     4141 aataaccttt caagagttat tgcagcagtt tctcatgaac taatcacatc aaccaccaga
     4201 gcactcacat ttggatgctg tgaagctttg tgtcttcttt ccactgcctt cccagtttgc
     4261 atttggagtt taggttggca ctgtggagtg cctccactga gtgcctcaga tgagtctagg
     4321 aagagctgta ccgttgggat ggccacaatg attctgaccc tgctctcgtc agcttggttc
     4381 ccattggatc tctcagccca tcaagatgct ttgattttgg ccggaaactt gcttgcagcc
     4441 agtgctccca aatctctgag aagttcatgg gcctctgaag aagaagccaa cccagcagcc
     4501 accaagcaag aggaggtctg gccagccctg ggggaccggg ccctggtgcc catggtggag
     4561 cagctcttct ctcacctgct gaaggtgatt aacatttgtg cccacgtcct ggatgacgtg
     4621 gctcctggac ccgcaataaa ggcagccttg ccttctctaa caaacccccc ttctctaagt
     4681 cccatccgac gaaaggggaa ggagaaagaa ccaggagaac aagcatctgt accgttgagt
     4741 cccaagaaag gcagtgaggc cagtgcagct tctagacaat ctgatacctc aggtcctgtt
     4801 acaacaagta aatcctcatc actggggagt ttctatcatc ttccttcata cctcaaactg
     4861 catgatgtcc tgaaagctac acacgctaac tacaaggtca cgctggatct tcagaacagc
     4921 acggaaaagt ttggagggtt tcttcgctca gccttggatg ttctttctca gatactagag
     4981 ctggccacac tgcaggacat tgggaagtgt gttgaagaga tcctaggata cctgaaatcc
     5041 tgctttagtc gagaaccaat gatggcaact gtttgtgttc aacaattgtt gaagactctc
     5101 tttggcacaa acttggcctc ccagtttgat ggcttatctt ccaaccccag caagtcacaa
     5161 ggccgagcac agcgccttgg ctcctccagt gtgaggccag gcttgtacca ctactgcttc
     5221 atggccccgt acacccactt cacccaggcc ctcgctgacg ccagcctgag gaacatggtg
     5281 caggcggagc aggagaacga cacctcggga tggtttgatg tcctccagaa agtgtctacc
     5341 cagttgaaga caaacctcac gagtgtcaca aagaaccgtg cagataagaa tgctattcat
     5401 aatcacattc gtttgtttga acctcttgtt ataaaagctt taaaacagta cacgactaca
     5461 acatgtgtgc agttacagaa gcaggtttta gatttgctgg cgcagctggt tcagttacgg
     5521 gttaattact gtcttctgga ttcagatcag gtgtttattg gctttgtatt gaaacagttt
     5581 gaatacattg aagtgggcca gttcagggaa tcagaggcaa tcattccaaa catctttttc
     5641 ttcttggtat tactatctta tgaacgctat cattcaaaac agatcattgg aattcctaaa
     5701 atcattcagc tctgtgatgg catcatggcc agtggaagga aggctgtgac acatgccata
     5761 ccggctctgc agcccatagt ccacgacctc tttgtattaa gaggaacaaa taaagctgat
     5821 gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt caatgttact gagactcatc
     5881 cagtaccatc aggtgttgga gatgttcatt cttgtcctgc agcagtgcca caaggagaat
     5941 gaagacaagt ggaagcgact gtctcgacag atagctgaca tcatcctccc aatgttagcc
     6001 aaacagcaga tgcacattga ctctcatgaa gcccttggag tgttaaatac attatttgag
     6061 attttggccc cttcctccct ccgtccggta gacatgcttt tacggagtat gttcgtcact
     6121 ccaaacacaa tggcgtccgt gagcactgtt caactgtgga tatcgggaat tctggccatt
     6181 ttgagggttc tgatttccca gtcaactgaa gatattgttc tttctcgtat tcaggagctc
     6241 tccttctctc cgtatttaat ctcctgtaca gtaattaata ggttaagaga tggggacagt
     6301 acttcaacgc tagaagaaca cagtgaaggg aaacaaataa agaatttgcc agaagaaaca
     6361 ttttcaaggt ttctattaca actggttggt attcttttag aagacattgt tacaaaacag
     6421 ctgaaggtgg aaatgagtga gcagcaacat actttctatt gccaggaact aggcacactg
     6481 ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc ggagaatcac agcagctgcc
     6541 actaggctgt tccgcagtga tggctgtggc ggcagtttct acaccctgga cagcttgaac
     6601 ttgcgggctc gttccatgat caccacccac ccggccctgg tgctgctctg gtgtcagata
     6661 ctgctgcttg tcaaccacac cgactaccgc tggtgggcag aagtgcagca gaccccgaaa
     6721 agacacagtc tgtccagcac aaagttactt agtccccaga tgtctggaga agaggaggat
     6781 tctgacttgg cagccaaact tggaatgtgc aatagagaaa tagtacgaag aggggctctc
     6841 attctcttct gtgattatgt ctgtcagaac ctccatgact ccgagcactt aacgtggctc
     6901 attgtaaatc acattcaaga tctgatcagc ctttcccacg agcctccagt acaggacttc
     6961 atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt tcatccaggc aattcagtct
     7021 cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa ctcttcagtg cttggagggg
     7081 atccatctca gccagtcggg agctgtgctc acgctgtatg tggacaggct tctgtgcacc
     7141 cctttccgtg tgctggctcg catggtcgac atccttgctt gtcgccgggt agaaatgctt
     7201 ctggctgcaa atttacagag cagcatggcc cagttgccaa tggaagaact caacagaatc
     7261 caggaatacc ttcagagcag cgggctcgct cagagacacc aaaggctcta ttccctgctg
     7321 gacaggtttc gtctctccac catgcaagac tcacttagtc cctctcctcc agtctcttcc
     7381 cacccgctgg acggggatgg gcacgtgtca ctggaaacag tgagtccgga caaagactgg
     7441 tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag attctgcact gctggaaggt
     7501 gcagagctgg tgaatcggat tcctgctgaa gatatgaatg ccttcatgat gaactcggag
     7561 ttcaacctaa gcctgctagc tccatgctta agcctaggga tgagtgaaat ttctggtggc
     7621 cagaagagtg ccctttttga agcagcccgt gaggtgactc tggcccgtgt gagcggcacc
     7681 gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg agctgcctgc agaaccggcg
     7741 gcctactgga gcaagttgaa tgatctgttt ggggatgctg cactgtatca gtccctgccc
     7801 actctggccc gggccctggc acagtacctg gtggtggtct ccaaactgcc cagtcatttg
     7861 caccttcctc ctgagaaaga gaaggacatt gtgaaattcg tggtggcaac ccttgaggcc
     7921 ctgtcctggc atttgatcca tgagcagatc ccgctgagtc tggatctcca ggcagggctg
     7981 gactgctgct gcctggccct gcagctgcct ggcctctgga gcgtggtctc ctccacagag
     8041 tttgtgaccc acgcctgctc cctcatccac tgtgtgcact tcatcctgga ggccgttgca
     8101 gtgcagcctg gagagcagct tcttagtcca gaaagaagga caaatacccc aaaagccatc
     8161 agcgaggagg aggaggaagt agatccaaac acacagaatc ctaagtatat cactgcagcc
     8221 tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg tgttggcctt gggtcataaa
     8281 aggaatagcg gcgtgccggc gtttctcacg ccattgctca ggaacatcat catcagcctg
     8341 gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc cactggtgtg gaagcttgga
     8401 tggtcaccca aaccgggagg ggattttggc acagcattcc ctgagatccc cgtggagttc
     8461 ctccaggaaa aggaagtctt taaggagttc atctaccgca tcaacacact aggctggacc
     8521 agtcgtactc agtttgaaga aacttgggcc accctccttg gtgtcctggt gacgcagccc
     8581 ctcgtgatgg agcaggagga gagcccacca gaagaagaca cagagaggac ccagatcaac
     8641 gtcctggccg tgcaggccat cacctcactg gtgctcagtg caatgactgt gcctgtggcc
     8701 ggcaacccag ctgtaagctg cttggagcag cagccccgga acaagcctct gaaagctctc
     8761 gacaccaggt ttgggaggaa gctgagcatt atcagaggga ttgtggagca agagattcaa
     8821 gcaatggttt caaagagaga gaatattgcc acccatcatt tatatcaggc atgggatcct
     8881 gtcccttctc tgtctccggc tactacaggt gccctcatca gccacgagaa gctgctgctg
     8941 cagatcaacc ccgagcggga gctggggagc atgagctaca aactcggcca ggtgtccata
     9001 cactccgtgt ggctagggaa cagcatcaca cccctgaggg aggaggaatg ggacgaggaa
     9061 gaggaggagg aggccgacgc ccctgcacct tcgtcaccac ccacgtctcc agtcaactcc
     9121 aggaaacacc gggctggagt tgacatccac tcctgttcgc agtttttgct tgagttgtac
     9181 agccgctgga tcctgccgtc cagctcagcc aggaggaccc cggccatcct gatcagtgag
     9241 gtggtcagat cccttctagt ggtctcagac ttgttcaccg agcgcaacca gtttgagctg
     9301 atgtatgtga cgctgacaga actgcgaagg gtgcaccctt cagaagacga gatcctcgct
     9361 cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc ttgggatgga caaggccgtg
     9421 gcggagcctg tcagccgcct gctggagagc acgctcagga gcagccacct gcccagcagg
     9481 gttggagccc tgcacggcat cctctatgtg ctggagtgcg acctgctgga cgacactgcc
     9541 aagcagctca tcccggtcat cagcgactat ctcctctcca acctgaaagg gatcgcccac
     9601 tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt gtgccactgc gttttacctc
     9661 attgagaact atcctctgga cgtagggccg gaattttcag catcaataat acagatgtgt
     9721 ggggtgatgc tgtctggaag tgaggagtcc accccctcca tcatttacca ctgtgccctc
     9781 agaggcctgg agcgcctcct gctctctgag cagctctccc gcctggatgc agaatcgctg
     9841 gtcaagctga gtgtggacag agtgaacgtg cacagcccgc accgggccat ggcggctctg
     9901 ggcctgatgc tcacctgcat gtacacagga aaggagaaag tcagtccggg tagaacttca
     9961 gaccctaatc ctgcagcccc cgacagcgag tcagtgattg ttgctatgga gcgggtatct
    10021 gttctttttg ataggatcag gaaaggcttt ccttgtgaag ccagagtggt ggccaggatc
    10081 ctgccccagt ttctagacga cttcttccca ccccaggaca tcatgaacaa agtcatcgga
    10141 gagtttctgt ccaaccagca gccatacccc cagttcatgg ccaccgtggt gtataaggtg
    10201 tttcagactc tgcacagcac cgggcagtcg tccatggtcc gggactgggt catgctgtcc
    10261 ctctccaact tcacgcagag ggccccggtc gccatggcca cgtggagcct ctcctgcttc
    10321 tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc tcccacatgt catcagcagg
    10381 atgggcaagc tggagcaggt ggacgtgaac cttttctgcc tggtcgccac agacttctac
    10441 agacaccaga tagaggagga gctcgaccgc agggccttcc agtctgtgct tgaggtggtt
    10501 gcagccccag gaagcccata tcaccggctg ctgacttgtt tacgaaatgt ccacaaggtc
    10561 accacctgct aaaacctcta cttccaatcg caccatcatc accatcacca tcaccaccat
    10621 gattacaagg atgacgacga taagtgaggc ggccgccaac ttgatatcaa gcttatcgat
    10681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
    10741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
    10801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
    10861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
    10921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
    10981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
    11041 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
    11101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    11161 aatccagcgg accttccttc ccgcgggcgc ccccctcctc acggcgagcg aagcttgtcg
    11221 agaagtacta gaggatctct agagcctgca gtctcgacaa gcttgtcgag aagtactaga
    11281 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    11341 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    11401 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    11461 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    11521 tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta gttccaaact
    11581 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
    11641 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
    11701 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
    11761 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
    11821 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
    11881 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    11941 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    12001 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    12061 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    12121 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    12181 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    12241 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    12301 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
    12361 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    12421 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    12481 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    12541 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    12601 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    12661 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    12721 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    12781 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    12841 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    12901 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    12961 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    13021 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    13081 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    13141 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    13201 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    13261 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    13321 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    13381 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    13441 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13501 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13561 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13621 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    13681 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13741 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    13801 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    13861 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    13921 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    13981 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    14041 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    14101 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    14161 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    14221 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    14281 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    14341 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
    14401 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
    14461 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
    14521 aaaagcatac tggacttttg ttatggctaa agcaaactct tcattttctg aagtgcaaat
    14581 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt
    14641 tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt
    14701 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
    14761 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
    14821 ccaagcgcgt tggcctcatg cttcaagaga ttgatgagcg cggtggcaat gccctgcctc
    14881 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
    14941 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
    15001 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
    15061 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
    15121 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
    15181 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct
    15241 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
    15301 catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
    15361 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta
    15421 cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc gtttccacgg
    15481 tgtgcgtcac ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc
    15541 gaacgagcgc aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt
    15601 ctacggcaag gtgctgtgca cggatctgcc cttgcttcag gagatcggta gacctcggcc
    15661 gtcgcggcgc ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct
    15721 ggaaggcgag catcgtttgt tcgcccagga ctctagctat agttctagtg gttggctacg
    15781 tacccgtagt ggctatggca gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt
    15841 cacccgaact tgggggttgg ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat
    15901 ggggtctcgg tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttat
//
