LOCUS       sequence-247719-       16119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16119)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 16119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..16119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        30..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        410..613
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     564..584
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        660..769
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             804..827
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9925..9934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10629..10658
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10659..10682
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10772..10792)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11190..11201)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11426..11560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11463..11482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11517..11536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11589..11754
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      11938..12392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12025..12044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12234..12255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12418..12522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12523..12591,12592..13383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12741..12760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13554..14142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14043..14062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14296..14313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  14447..14671
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(14738..15271)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(15460..15465,15466..15482,15483..15488))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    15996..16044
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     16069..16088
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cggatctgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      601 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaatacgg
      661 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
      721 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca ctcgacggat
      781 ccttaagaag gagatatact atggactaca aagacgatga cgacaagatg gcgaccctgg
      841 aaaagctgat gaaggccttc gagtccctca agtccttcca acagcagcaa cagcaacaac
      901 agcagcaaca gcaacaacag cagcaacagc aacaacagca gcaacagcaa caacagcagc
      961 aacagcaaca acagcagcaa cagcaacaac agcagcaaca gcaacaacag cagcaacagc
     1021 aacaacagca gcaacagcaa caacagcagc aacagcaaca acagcagcaa cagcaacaac
     1081 agcagcaaca gcaacaacaa cagcagcaac agcaacaaca gcagcaacag caacagcagc
     1141 agcaacagca acaacagcag caacagcaac aacagcagca acagcaacaa cagcagcaac
     1201 agcaacaaca gcagcaacag caacaacagc agcaacagca acaacagcag caacagcaac
     1261 aacagcagca acagcaacaa cagcagcaac agcaaccgcc accgccgccg ccgccgccgc
     1321 cgcctcctca gcttcctcag ccgccgccgc aggcacagcc gctgctgcct cagccgcagc
     1381 cgcccccgcc gccgcccccg ccgccacccg gcccggctgt ggctgaggag ccgctgcacc
     1441 gaccaaagaa agaactttca gctaccaaga aagaccgtgt gaatcattgt ctgacaatat
     1501 gtgaaaacat agtggcacag tctgtcagaa attctccaga atttcagaaa cttctgggca
     1561 tcgctatgga actttttctg ctgtgcagtg atgacgcaga gtcagatgtc aggatggtgg
     1621 ctgacgaatg cctcaacaaa gttatcaaag ctttgatgga ttctaatctt ccaaggttac
     1681 agctcgagct ctataaggaa attaaaaaga atggtgcccc tcggagtttg cgtgctgccc
     1741 tgtggaggtt tgctgagctg gctcacctgg ttcggcctca gaaatgcagg ccttacctgg
     1801 tgaaccttct gccgtgcctg actcgaacaa gcaagagacc cgaagaatca gtccaggaga
     1861 ccttggctgc agctgttccc aaaattatgg cttcttttgg caattttgca aatgacaatg
     1921 aaattaaggt tttgttaaag gccttcatag cgaacctgaa gtcaagctcc cccaccattc
     1981 ggcggacagc ggctggatca gcagtgagca tctgccagca ctcaagaagg acacaatatt
     2041 tctatagttg gctactaaat gtgctcttag gcttactcgt tcctgtcgag gatgaacact
     2101 ccactctgct gattcttggc gtgctgctca ccctgaggta tttggtgccc ttgctgcagc
     2161 agcaggtcaa ggacacaagc ctgaaaggca gcttcggagt gacaaggaaa gaaatggaag
     2221 tctctccttc tgcagagcag cttgtccagg tttatgaact gacgttacat catacacagc
     2281 accaagacca caatgttgtg accggagccc tggagctgtt gcagcagctc ttcagaacgc
     2341 ctccacccga gcttctgcaa accctgaccg cagtcggggg cattgggcag ctcaccgctg
     2401 ctaaggagga gtctggtggc cgaagccgta gtgggagtat tgtggaactt atagctggag
     2461 ggggttcctc atgcagccct gtcctttcaa gaaaacaaaa aggcaaagtg ctcttaggag
     2521 aagaagaagc cttggaggat gactctgaat cgagatcgga tgtcagcagc tctgccttaa
     2581 cagcctcagt gaaggatgag atcagtggag agctggctgc ttcttcaggg gtttccactc
     2641 cagggtcagc aggtcatgac atcatcacag aacagccacg gtcacagcac acactgcagg
     2701 cggactcagt ggatctggcc agctgtgact tgacaagctc tgccactgat ggggatgagg
     2761 aggatatctt gagccacagc tccagccagg tcagcgccgt cccatctgac cctgccatgg
     2821 acctgaatga tgggacccag gcctcgtcgc ccatcagcga cagctcccag accaccaccg
     2881 aagggcctga ttcagctgtt accccttcag acagttctga aattgtgtta gacggtaccg
     2941 acaaccagta tttgggcctg cagattggac agccccagga tgaagatgag gaagccacag
     3001 gtattcttcc tgatgaagcc tcggaggcct tcaggaactc ttccatggcc cttcaacagg
     3061 cacatttatt gaaaaacatg agtcactgca ggcagccttc tgacagcagt gttgataaat
     3121 ttgtgttgag agatgaagct actgaaccgg gtgatcaaga aaacaagcct tgccgcatca
     3181 aaggtgacat tggacagtcc actgatgatg actctgcacc tcttgtccat tgtgtccgcc
     3241 ttttatctgc ttcgtttttg ctaacagggg gaaaaaatgt gctggttccg gacagggatg
     3301 tgagggtcag cgtgaaggcc ctggccctca gctgtgtggg agcagctgtg gccctccacc
     3361 cggaatcttt cttcagcaaa ctctataaag ttcctcttga caccacggaa taccctgagg
     3421 aacagtatgt ctcagacatc ttgaactaca tcgatcatgg agacccacag gttcgaggag
     3481 ccactgccat tctctgtggg accctcatct gctccatcct cagcaggtcc cgcttccacg
     3541 tgggagattg gatgggcacc attagaaccc tcacaggaaa tacattttct ttggcggatt
     3601 gcattccttt gctgcggaaa acactgaagg atgagtcttc tgttacttgc aagttagctt
     3661 gtacagctgt gaggaactgt gtcatgagtc tctgcagcag cagctacagt gagttaggac
     3721 tgcagctgat catcgatgtg ctgactctga ggaacagttc ctattggctg gtgaggacag
     3781 agcttctgga aacccttgca gagattgact tcaggctggt gagctttttg gaggcaaaag
     3841 cagaaaactt acacagaggg gctcatcatt atacagggct tttaaaactg caagaacgag
     3901 tgctcaataa tgttgtcatc catttgcttg gagatgaaga ccccagggtg cgacatgttg
     3961 ccgcagcatc actaattagg cttgtcccaa agctgtttta taaatgtgac caaggacaag
     4021 ctgatccagt agtggccgtg gcaagagatc aaagcagtgt ttacctgaaa cttctcatgc
     4081 atgagacgca gcctccatct catttctccg tcagcacaat aaccagaata tatagaggct
     4141 ataacctact accaagcata acagacgtca ctatggaaaa taacctttca agagttattg
     4201 cagcagtttc tcatgaacta atcacatcaa ccaccagagc actcacattt ggatgctgtg
     4261 aagctttgtg tcttctttcc actgccttcc cagtttgcat ttggagttta ggttggcact
     4321 gtggagtgcc tccactgagt gcctcagatg agtctaggaa gagctgtacc gttgggatgg
     4381 ccacaatgat tctgaccctg ctctcgtcag cttggttccc attggatctc tcagcccatc
     4441 aagatgcttt gattttggcc ggaaacttgc ttgcagccag tgctcccaaa tctctgagaa
     4501 gttcatgggc ctctgaagaa gaagccaacc cagcagccac caagcaagag gaggtctggc
     4561 cagccctggg ggaccgggcc ctggtgccca tggtggagca gctcttctct cacctgctga
     4621 aggtgattaa catttgtgcc cacgtcctgg atgacgtggc tcctggaccc gcaataaagg
     4681 cagccttgcc ttctctaaca aacccccctt ctctaagtcc catccgacga aaggggaagg
     4741 agaaagaacc aggagaacaa gcatctgtac cgttgagtcc caagaaaggc agtgaggcca
     4801 gtgcagcttc tagacaatct gatacctcag gtcctgttac aacaagtaaa tcctcatcac
     4861 tggggagttt ctatcatctt ccttcatacc tcaaactgca tgatgtcctg aaagctacac
     4921 acgctaacta caaggtcacg ctggatcttc agaacagcac ggaaaagttt ggagggtttc
     4981 ttcgctcagc cttggatgtt ctttctcaga tactagagct ggccacactg caggacattg
     5041 ggaagtgtgt tgaagagatc ctaggatacc tgaaatcctg ctttagtcga gaaccaatga
     5101 tggcaactgt ttgtgttcaa caattgttga agactctctt tggcacaaac ttggcctccc
     5161 agtttgatgg cttatcttcc aaccccagca agtcacaagg ccgagcacag cgccttggct
     5221 cctccagtgt gaggccaggc ttgtaccact actgcttcat ggccccgtac acccacttca
     5281 cccaggccct cgctgacgcc agcctgagga acatggtgca ggcggagcag gagaacgaca
     5341 cctcgggatg gtttgatgtc ctccagaaag tgtctaccca gttgaagaca aacctcacga
     5401 gtgtcacaaa gaaccgtgca gataagaatg ctattcataa tcacattcgt ttgtttgaac
     5461 ctcttgttat aaaagcttta aaacagtaca cgactacaac atgtgtgcag ttacagaagc
     5521 aggttttaga tttgctggcg cagctggttc agttacgggt taattactgt cttctggatt
     5581 cagatcaggt gtttattggc tttgtattga aacagtttga atacattgaa gtgggccagt
     5641 tcagggaatc agaggcaatc attccaaaca tctttttctt cttggtatta ctatcttatg
     5701 aacgctatca ttcaaaacag atcattggaa ttcctaaaat cattcagctc tgtgatggca
     5761 tcatggccag tggaaggaag gctgtgacac atgccatacc ggctctgcag cccatagtcc
     5821 acgacctctt tgtattaaga ggaacaaata aagctgatgc aggaaaagag cttgaaaccc
     5881 aaaaagaggt ggtggtgtca atgttactga gactcatcca gtaccatcag gtgttggaga
     5941 tgttcattct tgtcctgcag cagtgccaca aggagaatga agacaagtgg aagcgactgt
     6001 ctcgacagat agctgacatc atcctcccaa tgttagccaa acagcagatg cacattgact
     6061 ctcatgaagc ccttggagtg ttaaatacat tatttgagat tttggcccct tcctccctcc
     6121 gtccggtaga catgctttta cggagtatgt tcgtcactcc aaacacaatg gcgtccgtga
     6181 gcactgttca actgtggata tcgggaattc tggccatttt gagggttctg atttcccagt
     6241 caactgaaga tattgttctt tctcgtattc aggagctctc cttctctccg tatttaatct
     6301 cctgtacagt aattaatagg ttaagagatg gggacagtac ttcaacgcta gaagaacaca
     6361 gtgaagggaa acaaataaag aatttgccag aagaaacatt ttcaaggttt ctattacaac
     6421 tggttggtat tcttttagaa gacattgtta caaaacagct gaaggtggaa atgagtgagc
     6481 agcaacatac tttctattgc caggaactag gcacactgct aatgtgtctg atccacatct
     6541 tcaagtctgg aatgttccgg agaatcacag cagctgccac taggctgttc cgcagtgatg
     6601 gctgtggcgg cagtttctac accctggaca gcttgaactt gcgggctcgt tccatgatca
     6661 ccacccaccc ggccctggtg ctgctctggt gtcagatact gctgcttgtc aaccacaccg
     6721 actaccgctg gtgggcagaa gtgcagcaga ccccgaaaag acacagtctg tccagcacaa
     6781 agttacttag tccccagatg tctggagaag aggaggattc tgacttggca gccaaacttg
     6841 gaatgtgcaa tagagaaata gtacgaagag gggctctcat tctcttctgt gattatgtct
     6901 gtcagaacct ccatgactcc gagcacttaa cgtggctcat tgtaaatcac attcaagatc
     6961 tgatcagcct ttcccacgag cctccagtac aggacttcat cagtgccgtt catcggaact
     7021 ctgctgccag cggcctgttc atccaggcaa ttcagtctcg ttgtgaaaac ctttcaactc
     7081 caaccatgct gaagaaaact cttcagtgct tggaggggat ccatctcagc cagtcgggag
     7141 ctgtgctcac gctgtatgtg gacaggcttc tgtgcacccc tttccgtgtg ctggctcgca
     7201 tggtcgacat ccttgcttgt cgccgggtag aaatgcttct ggctgcaaat ttacagagca
     7261 gcatggccca gttgccaatg gaagaactca acagaatcca ggaatacctt cagagcagcg
     7321 ggctcgctca gagacaccaa aggctctatt ccctgctgga caggtttcgt ctctccacca
     7381 tgcaagactc acttagtccc tctcctccag tctcttccca cccgctggac ggggatgggc
     7441 acgtgtcact ggaaacagtg agtccggaca aagactggta cgttcatctt gtcaaatccc
     7501 agtgttggac caggtcagat tctgcactgc tggaaggtgc agagctggtg aatcggattc
     7561 ctgctgaaga tatgaatgcc ttcatgatga actcggagtt caacctaagc ctgctagctc
     7621 catgcttaag cctagggatg agtgaaattt ctggtggcca gaagagtgcc ctttttgaag
     7681 cagcccgtga ggtgactctg gcccgtgtga gcggcaccgt gcagcagctc cctgctgtcc
     7741 atcatgtctt ccagcccgag ctgcctgcag aaccggcggc ctactggagc aagttgaatg
     7801 atctgtttgg ggatgctgca ctgtatcagt ccctgcccac tctggcccgg gccctggcac
     7861 agtacctggt ggtggtctcc aaactgccca gtcatttgca ccttcctcct gagaaagaga
     7921 aggacattgt gaaattcgtg gtggcaaccc ttgaggccct gtcctggcat ttgatccatg
     7981 agcagatccc gctgagtctg gatctccagg cagggctgga ctgctgctgc ctggccctgc
     8041 agctgcctgg cctctggagc gtggtctcct ccacagagtt tgtgacccac gcctgctccc
     8101 tcatccactg tgtgcacttc atcctggagg ccgttgcagt gcagcctgga gagcagcttc
     8161 ttagtccaga aagaaggaca aataccccaa aagccatcag cgaggaggag gaggaagtag
     8221 atccaaacac acagaatcct aagtatatca ctgcagcctg tgagatggtg gcagaaatgg
     8281 tggagtctct gcagtcggtg ttggccttgg gtcataaaag gaatagcggc gtgccggcgt
     8341 ttctcacgcc attgctcagg aacatcatca tcagcctggc ccgcctgccc cttgtcaaca
     8401 gctacacacg tgtgccccca ctggtgtgga agcttggatg gtcacccaaa ccgggagggg
     8461 attttggcac agcattccct gagatccccg tggagttcct ccaggaaaag gaagtcttta
     8521 aggagttcat ctaccgcatc aacacactag gctggaccag tcgtactcag tttgaagaaa
     8581 cttgggccac cctccttggt gtcctggtga cgcagcccct cgtgatggag caggaggaga
     8641 gcccaccaga agaagacaca gagaggaccc agatcaacgt cctggccgtg caggccatca
     8701 cctcactggt gctcagtgca atgactgtgc ctgtggccgg caacccagct gtaagctgct
     8761 tggagcagca gccccggaac aagcctctga aagctctcga caccaggttt gggaggaagc
     8821 tgagcattat cagagggatt gtggagcaag agattcaagc aatggtttca aagagagaga
     8881 atattgccac ccatcattta tatcaggcat gggatcctgt cccttctctg tctccggcta
     8941 ctacaggtgc cctcatcagc cacgagaagc tgctgctgca gatcaacccc gagcgggagc
     9001 tggggagcat gagctacaaa ctcggccagg tgtccataca ctccgtgtgg ctagggaaca
     9061 gcatcacacc cctgagggag gaggaatggg acgaggaaga ggaggaggag gccgacgccc
     9121 ctgcaccttc gtcaccaccc acgtctccag tcaactccag gaaacaccgg gctggagttg
     9181 acatccactc ctgttcgcag tttttgcttg agttgtacag ccgctggatc ctgccgtcca
     9241 gctcagccag gaggaccccg gccatcctga tcagtgaggt ggtcagatcc cttctagtgg
     9301 tctcagactt gttcaccgag cgcaaccagt ttgagctgat gtatgtgacg ctgacagaac
     9361 tgcgaagggt gcacccttca gaagacgaga tcctcgctca gtacctggtg cctgccacct
     9421 gcaaggcagc tgccgtcctt gggatggaca aggccgtggc ggagcctgtc agccgcctgc
     9481 tggagagcac gctcaggagc agccacctgc ccagcagggt tggagccctg cacggcatcc
     9541 tctatgtgct ggagtgcgac ctgctggacg acactgccaa gcagctcatc ccggtcatca
     9601 gcgactatct cctctccaac ctgaaaggga tcgcccactg cgtgaacatt cacagccagc
     9661 agcacgtact ggtcatgtgt gccactgcgt tttacctcat tgagaactat cctctggacg
     9721 tagggccgga attttcagca tcaataatac agatgtgtgg ggtgatgctg tctggaagtg
     9781 aggagtccac cccctccatc atttaccact gtgccctcag aggcctggag cgcctcctgc
     9841 tctctgagca gctctcccgc ctggatgcag aatcgctggt caagctgagt gtggacagag
     9901 tgaacgtgca cagcccgcac cgggccatgg cggctctggg cctgatgctc acctgcatgt
     9961 acacaggaaa ggagaaagtc agtccgggta gaacttcaga ccctaatcct gcagcccccg
    10021 acagcgagtc agtgattgtt gctatggagc gggtatctgt tctttttgat aggatcagga
    10081 aaggctttcc ttgtgaagcc agagtggtgg ccaggatcct gccccagttt ctagacgact
    10141 tcttcccacc ccaggacatc atgaacaaag tcatcggaga gtttctgtcc aaccagcagc
    10201 cataccccca gttcatggcc accgtggtgt ataaggtgtt tcagactctg cacagcaccg
    10261 ggcagtcgtc catggtccgg gactgggtca tgctgtccct ctccaacttc acgcagaggg
    10321 ccccggtcgc catggccacg tggagcctct cctgcttctt tgtcagcgcg tccaccagcc
    10381 cgtgggtcgc ggcgatcctc ccacatgtca tcagcaggat gggcaagctg gagcaggtgg
    10441 acgtgaacct tttctgcctg gtcgccacag acttctacag acaccagata gaggaggagc
    10501 tcgaccgcag ggccttccag tctgtgcttg aggtggttgc agccccagga agcccatatc
    10561 accggctgct gacttgttta cgaaatgtcc acaaggtcac cacctgctaa aacctctact
    10621 tccaatcgca ccatcatcac catcaccatc accaccatga ttacaaggat gacgacgata
    10681 agtgaggcgg ccgccaactt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
    10741 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    10801 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    10861 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    10921 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    10981 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    11041 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    11101 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    11161 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    11221 gcgggcgccc ccctcctcac ggcgagcgaa gcttgtcgag aagtactaga ggatctctag
    11281 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
    11341 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    11401 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    11461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    11521 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
    11581 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg
    11641 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc
    11701 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg
    11761 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt
    11821 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat
    11881 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgggacg
    11941 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
    12001 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
    12061 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
    12121 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
    12181 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta atagtggact
    12241 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    12301 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    12361 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
    12421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    12481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    12541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    12601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
    12661 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
    12721 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
    12781 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
    12841 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
    12901 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    12961 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    13021 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
    13081 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    13141 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    13201 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    13261 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    13321 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    13381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    13441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    13501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    13561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    13621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    13681 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    13741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    13801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    13861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    13921 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    13981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    14041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    14101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    14161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    14221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    14281 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
    14341 tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg taacctggca
    14401 aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg ggcggacaat
    14461 aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta aactagacag
    14521 aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg gacttttgtt
    14581 atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat taaagagggg
    14641 cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta ccgaacaact
    14701 ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta cttgggtcga
    14761 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
    14821 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
    14881 tcaagagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
    14941 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
    15001 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
    15061 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
    15121 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
    15181 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
    15241 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
    15301 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
    15361 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
    15421 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
    15481 tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg
    15541 ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa ggtttcggtc
    15601 tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt gctgtgcacg
    15661 gatctgccct tgcttcagga gatcggtaga cctcggccgt cgcggcgctt gccggtggtg
    15721 ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc
    15781 gcccaggact ctagctatag ttctagtggt tggctacgta cccgtagtgg ctatggcagg
    15841 gcttgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg ggggttgggg
    15901 tggggaaaag gaagaaacgc gggcgtattg gtcccaatgg ggtctcggtg gggtatcgac
    15961 agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa cacccgtgcg
    16021 ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
    16081 gtgtttcagt tagcctcccc catctcccgg taccgcatg
//
