LOCUS       sequence-247722-       15563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intermediate vector containing HTT gene, except for polyQ region. 
            Used to clone various polyQ lengths into HTT sequence with N-term 
            FLAG tag. Baculovirus transfer vector for insect and mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N entry vector
FEATURES             Location/Qualifiers
     source          1..15563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        30..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        410..613
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     564..584
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        660..769
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             804..827
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9369..9378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10073..10102
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10103..10126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10216..10236)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10634..10645)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10870..11004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10907..10926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10961..10980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  11033..11198
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      11382..11836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11469..11488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11678..11699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11862..11966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11967..12035,12036..12827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12185..12204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12998..13586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13487..13506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13740..13757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  13891..14115
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(14182..14715)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(14904..14909,14910..14926,14927..14932))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    15440..15488
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     15513..15532
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cggatctgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      601 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaatacgg
      661 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
      721 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca ctcgacggat
      781 ccttaagaag gagatatact atggactaca aagacgatga cgacaagatg gcgaccctgg
      841 aaaagcgctg accttagtcg ctaacctgca ggagccgctg caccgaccaa agaaagaact
      901 ttcagctacc aagaaagacc gtgtgaatca ttgtctgaca atatgtgaaa acatagtggc
      961 acagtctgtc agaaattctc cagaatttca gaaacttctg ggcatcgcta tggaactttt
     1021 tctgctgtgc agtgatgacg cagagtcaga tgtcaggatg gtggctgacg aatgcctcaa
     1081 caaagttatc aaagctttga tggattctaa tcttccaagg ttacagctcg agctctataa
     1141 ggaaattaaa aagaatggtg cccctcggag tttgcgtgct gccctgtgga ggtttgctga
     1201 gctggctcac ctggttcggc ctcagaaatg caggccttac ctggtgaacc ttctgccgtg
     1261 cctgactcga acaagcaaga gacccgaaga atcagtccag gagaccttgg ctgcagctgt
     1321 tcccaaaatt atggcttctt ttggcaattt tgcaaatgac aatgaaatta aggttttgtt
     1381 aaaggccttc atagcgaacc tgaagtcaag ctcccccacc attcggcgga cagcggctgg
     1441 atcagcagtg agcatctgcc agcactcaag aaggacacaa tatttctata gttggctact
     1501 aaatgtgctc ttaggcttac tcgttcctgt cgaggatgaa cactccactc tgctgattct
     1561 tggcgtgctg ctcaccctga ggtatttggt gcccttgctg cagcagcagg tcaaggacac
     1621 aagcctgaaa ggcagcttcg gagtgacaag gaaagaaatg gaagtctctc cttctgcaga
     1681 gcagcttgtc caggtttatg aactgacgtt acatcataca cagcaccaag accacaatgt
     1741 tgtgaccgga gccctggagc tgttgcagca gctcttcaga acgcctccac ccgagcttct
     1801 gcaaaccctg accgcagtcg ggggcattgg gcagctcacc gctgctaagg aggagtctgg
     1861 tggccgaagc cgtagtggga gtattgtgga acttatagct ggagggggtt cctcatgcag
     1921 ccctgtcctt tcaagaaaac aaaaaggcaa agtgctctta ggagaagaag aagccttgga
     1981 ggatgactct gaatcgagat cggatgtcag cagctctgcc ttaacagcct cagtgaagga
     2041 tgagatcagt ggagagctgg ctgcttcttc aggggtttcc actccagggt cagcaggtca
     2101 tgacatcatc acagaacagc cacggtcaca gcacacactg caggcggact cagtggatct
     2161 ggccagctgt gacttgacaa gctctgccac tgatggggat gaggaggata tcttgagcca
     2221 cagctccagc caggtcagcg ccgtcccatc tgaccctgcc atggacctga atgatgggac
     2281 ccaggcctcg tcgcccatca gcgacagctc ccagaccacc accgaagggc ctgattcagc
     2341 tgttacccct tcagacagtt ctgaaattgt gttagacggt accgacaacc agtatttggg
     2401 cctgcagatt ggacagcccc aggatgaaga tgaggaagcc acaggtattc ttcctgatga
     2461 agcctcggag gccttcagga actcttccat ggcccttcaa caggcacatt tattgaaaaa
     2521 catgagtcac tgcaggcagc cttctgacag cagtgttgat aaatttgtgt tgagagatga
     2581 agctactgaa ccgggtgatc aagaaaacaa gccttgccgc atcaaaggtg acattggaca
     2641 gtccactgat gatgactctg cacctcttgt ccattgtgtc cgccttttat ctgcttcgtt
     2701 tttgctaaca gggggaaaaa atgtgctggt tccggacagg gatgtgaggg tcagcgtgaa
     2761 ggccctggcc ctcagctgtg tgggagcagc tgtggccctc cacccggaat ctttcttcag
     2821 caaactctat aaagttcctc ttgacaccac ggaataccct gaggaacagt atgtctcaga
     2881 catcttgaac tacatcgatc atggagaccc acaggttcga ggagccactg ccattctctg
     2941 tgggaccctc atctgctcca tcctcagcag gtcccgcttc cacgtgggag attggatggg
     3001 caccattaga accctcacag gaaatacatt ttctttggcg gattgcattc ctttgctgcg
     3061 gaaaacactg aaggatgagt cttctgttac ttgcaagtta gcttgtacag ctgtgaggaa
     3121 ctgtgtcatg agtctctgca gcagcagcta cagtgagtta ggactgcagc tgatcatcga
     3181 tgtgctgact ctgaggaaca gttcctattg gctggtgagg acagagcttc tggaaaccct
     3241 tgcagagatt gacttcaggc tggtgagctt tttggaggca aaagcagaaa acttacacag
     3301 aggggctcat cattatacag ggcttttaaa actgcaagaa cgagtgctca ataatgttgt
     3361 catccatttg cttggagatg aagaccccag ggtgcgacat gttgccgcag catcactaat
     3421 taggcttgtc ccaaagctgt tttataaatg tgaccaagga caagctgatc cagtagtggc
     3481 cgtggcaaga gatcaaagca gtgtttacct gaaacttctc atgcatgaga cgcagcctcc
     3541 atctcatttc tccgtcagca caataaccag aatatataga ggctataacc tactaccaag
     3601 cataacagac gtcactatgg aaaataacct ttcaagagtt attgcagcag tttctcatga
     3661 actaatcaca tcaaccacca gagcactcac atttggatgc tgtgaagctt tgtgtcttct
     3721 ttccactgcc ttcccagttt gcatttggag tttaggttgg cactgtggag tgcctccact
     3781 gagtgcctca gatgagtcta ggaagagctg taccgttggg atggccacaa tgattctgac
     3841 cctgctctcg tcagcttggt tcccattgga tctctcagcc catcaagatg ctttgatttt
     3901 ggccggaaac ttgcttgcag ccagtgctcc caaatctctg agaagttcat gggcctctga
     3961 agaagaagcc aacccagcag ccaccaagca agaggaggtc tggccagccc tgggggaccg
     4021 ggccctggtg cccatggtgg agcagctctt ctctcacctg ctgaaggtga ttaacatttg
     4081 tgcccacgtc ctggatgacg tggctcctgg acccgcaata aaggcagcct tgccttctct
     4141 aacaaacccc ccttctctaa gtcccatccg acgaaagggg aaggagaaag aaccaggaga
     4201 acaagcatct gtaccgttga gtcccaagaa aggcagtgag gccagtgcag cttctagaca
     4261 atctgatacc tcaggtcctg ttacaacaag taaatcctca tcactgggga gtttctatca
     4321 tcttccttca tacctcaaac tgcatgatgt cctgaaagct acacacgcta actacaaggt
     4381 cacgctggat cttcagaaca gcacggaaaa gtttggaggg tttcttcgct cagccttgga
     4441 tgttctttct cagatactag agctggccac actgcaggac attgggaagt gtgttgaaga
     4501 gatcctagga tacctgaaat cctgctttag tcgagaacca atgatggcaa ctgtttgtgt
     4561 tcaacaattg ttgaagactc tctttggcac aaacttggcc tcccagtttg atggcttatc
     4621 ttccaacccc agcaagtcac aaggccgagc acagcgcctt ggctcctcca gtgtgaggcc
     4681 aggcttgtac cactactgct tcatggcccc gtacacccac ttcacccagg ccctcgctga
     4741 cgccagcctg aggaacatgg tgcaggcgga gcaggagaac gacacctcgg gatggtttga
     4801 tgtcctccag aaagtgtcta cccagttgaa gacaaacctc acgagtgtca caaagaaccg
     4861 tgcagataag aatgctattc ataatcacat tcgtttgttt gaacctcttg ttataaaagc
     4921 tttaaaacag tacacgacta caacatgtgt gcagttacag aagcaggttt tagatttgct
     4981 ggcgcagctg gttcagttac gggttaatta ctgtcttctg gattcagatc aggtgtttat
     5041 tggctttgta ttgaaacagt ttgaatacat tgaagtgggc cagttcaggg aatcagaggc
     5101 aatcattcca aacatctttt tcttcttggt attactatct tatgaacgct atcattcaaa
     5161 acagatcatt ggaattccta aaatcattca gctctgtgat ggcatcatgg ccagtggaag
     5221 gaaggctgtg acacatgcca taccggctct gcagcccata gtccacgacc tctttgtatt
     5281 aagaggaaca aataaagctg atgcaggaaa agagcttgaa acccaaaaag aggtggtggt
     5341 gtcaatgtta ctgagactca tccagtacca tcaggtgttg gagatgttca ttcttgtcct
     5401 gcagcagtgc cacaaggaga atgaagacaa gtggaagcga ctgtctcgac agatagctga
     5461 catcatcctc ccaatgttag ccaaacagca gatgcacatt gactctcatg aagcccttgg
     5521 agtgttaaat acattatttg agattttggc cccttcctcc ctccgtccgg tagacatgct
     5581 tttacggagt atgttcgtca ctccaaacac aatggcgtcc gtgagcactg ttcaactgtg
     5641 gatatcggga attctggcca ttttgagggt tctgatttcc cagtcaactg aagatattgt
     5701 tctttctcgt attcaggagc tctccttctc tccgtattta atctcctgta cagtaattaa
     5761 taggttaaga gatggggaca gtacttcaac gctagaagaa cacagtgaag ggaaacaaat
     5821 aaagaatttg ccagaagaaa cattttcaag gtttctatta caactggttg gtattctttt
     5881 agaagacatt gttacaaaac agctgaaggt ggaaatgagt gagcagcaac atactttcta
     5941 ttgccaggaa ctaggcacac tgctaatgtg tctgatccac atcttcaagt ctggaatgtt
     6001 ccggagaatc acagcagctg ccactaggct gttccgcagt gatggctgtg gcggcagttt
     6061 ctacaccctg gacagcttga acttgcgggc tcgttccatg atcaccaccc acccggccct
     6121 ggtgctgctc tggtgtcaga tactgctgct tgtcaaccac accgactacc gctggtgggc
     6181 agaagtgcag cagaccccga aaagacacag tctgtccagc acaaagttac ttagtcccca
     6241 gatgtctgga gaagaggagg attctgactt ggcagccaaa cttggaatgt gcaatagaga
     6301 aatagtacga agaggggctc tcattctctt ctgtgattat gtctgtcaga acctccatga
     6361 ctccgagcac ttaacgtggc tcattgtaaa tcacattcaa gatctgatca gcctttccca
     6421 cgagcctcca gtacaggact tcatcagtgc cgttcatcgg aactctgctg ccagcggcct
     6481 gttcatccag gcaattcagt ctcgttgtga aaacctttca actccaacca tgctgaagaa
     6541 aactcttcag tgcttggagg ggatccatct cagccagtcg ggagctgtgc tcacgctgta
     6601 tgtggacagg cttctgtgca cccctttccg tgtgctggct cgcatggtcg acatccttgc
     6661 ttgtcgccgg gtagaaatgc ttctggctgc aaatttacag agcagcatgg cccagttgcc
     6721 aatggaagaa ctcaacagaa tccaggaata ccttcagagc agcgggctcg ctcagagaca
     6781 ccaaaggctc tattccctgc tggacaggtt tcgtctctcc accatgcaag actcacttag
     6841 tccctctcct ccagtctctt cccacccgct ggacggggat gggcacgtgt cactggaaac
     6901 agtgagtccg gacaaagact ggtacgttca tcttgtcaaa tcccagtgtt ggaccaggtc
     6961 agattctgca ctgctggaag gtgcagagct ggtgaatcgg attcctgctg aagatatgaa
     7021 tgccttcatg atgaactcgg agttcaacct aagcctgcta gctccatgct taagcctagg
     7081 gatgagtgaa atttctggtg gccagaagag tgcccttttt gaagcagccc gtgaggtgac
     7141 tctggcccgt gtgagcggca ccgtgcagca gctccctgct gtccatcatg tcttccagcc
     7201 cgagctgcct gcagaaccgg cggcctactg gagcaagttg aatgatctgt ttggggatgc
     7261 tgcactgtat cagtccctgc ccactctggc ccgggccctg gcacagtacc tggtggtggt
     7321 ctccaaactg cccagtcatt tgcaccttcc tcctgagaaa gagaaggaca ttgtgaaatt
     7381 cgtggtggca acccttgagg ccctgtcctg gcatttgatc catgagcaga tcccgctgag
     7441 tctggatctc caggcagggc tggactgctg ctgcctggcc ctgcagctgc ctggcctctg
     7501 gagcgtggtc tcctccacag agtttgtgac ccacgcctgc tccctcatcc actgtgtgca
     7561 cttcatcctg gaggccgttg cagtgcagcc tggagagcag cttcttagtc cagaaagaag
     7621 gacaaatacc ccaaaagcca tcagcgagga ggaggaggaa gtagatccaa acacacagaa
     7681 tcctaagtat atcactgcag cctgtgagat ggtggcagaa atggtggagt ctctgcagtc
     7741 ggtgttggcc ttgggtcata aaaggaatag cggcgtgccg gcgtttctca cgccattgct
     7801 caggaacatc atcatcagcc tggcccgcct gccccttgtc aacagctaca cacgtgtgcc
     7861 cccactggtg tggaagcttg gatggtcacc caaaccggga ggggattttg gcacagcatt
     7921 ccctgagatc cccgtggagt tcctccagga aaaggaagtc tttaaggagt tcatctaccg
     7981 catcaacaca ctaggctgga ccagtcgtac tcagtttgaa gaaacttggg ccaccctcct
     8041 tggtgtcctg gtgacgcagc ccctcgtgat ggagcaggag gagagcccac cagaagaaga
     8101 cacagagagg acccagatca acgtcctggc cgtgcaggcc atcacctcac tggtgctcag
     8161 tgcaatgact gtgcctgtgg ccggcaaccc agctgtaagc tgcttggagc agcagccccg
     8221 gaacaagcct ctgaaagctc tcgacaccag gtttgggagg aagctgagca ttatcagagg
     8281 gattgtggag caagagattc aagcaatggt ttcaaagaga gagaatattg ccacccatca
     8341 tttatatcag gcatgggatc ctgtcccttc tctgtctccg gctactacag gtgccctcat
     8401 cagccacgag aagctgctgc tgcagatcaa ccccgagcgg gagctgggga gcatgagcta
     8461 caaactcggc caggtgtcca tacactccgt gtggctaggg aacagcatca cacccctgag
     8521 ggaggaggaa tgggacgagg aagaggagga ggaggccgac gcccctgcac cttcgtcacc
     8581 acccacgtct ccagtcaact ccaggaaaca ccgggctgga gttgacatcc actcctgttc
     8641 gcagtttttg cttgagttgt acagccgctg gatcctgccg tccagctcag ccaggaggac
     8701 cccggccatc ctgatcagtg aggtggtcag atcccttcta gtggtctcag acttgttcac
     8761 cgagcgcaac cagtttgagc tgatgtatgt gacgctgaca gaactgcgaa gggtgcaccc
     8821 ttcagaagac gagatcctcg ctcagtacct ggtgcctgcc acctgcaagg cagctgccgt
     8881 ccttgggatg gacaaggccg tggcggagcc tgtcagccgc ctgctggaga gcacgctcag
     8941 gagcagccac ctgcccagca gggttggagc cctgcacggc atcctctatg tgctggagtg
     9001 cgacctgctg gacgacactg ccaagcagct catcccggtc atcagcgact atctcctctc
     9061 caacctgaaa gggatcgccc actgcgtgaa cattcacagc cagcagcacg tactggtcat
     9121 gtgtgccact gcgttttacc tcattgagaa ctatcctctg gacgtagggc cggaattttc
     9181 agcatcaata atacagatgt gtggggtgat gctgtctgga agtgaggagt ccaccccctc
     9241 catcatttac cactgtgccc tcagaggcct ggagcgcctc ctgctctctg agcagctctc
     9301 ccgcctggat gcagaatcgc tggtcaagct gagtgtggac agagtgaacg tgcacagccc
     9361 gcaccgggcc atggcggctc tgggcctgat gctcacctgc atgtacacag gaaaggagaa
     9421 agtcagtccg ggtagaactt cagaccctaa tcctgcagcc cccgacagcg agtcagtgat
     9481 tgttgctatg gagcgggtat ctgttctttt tgataggatc aggaaaggct ttccttgtga
     9541 agccagagtg gtggccagga tcctgcccca gtttctagac gacttcttcc caccccagga
     9601 catcatgaac aaagtcatcg gagagtttct gtccaaccag cagccatacc cccagttcat
     9661 ggccaccgtg gtgtataagg tgtttcagac tctgcacagc accgggcagt cgtccatggt
     9721 ccgggactgg gtcatgctgt ccctctccaa cttcacgcag agggccccgg tcgccatggc
     9781 cacgtggagc ctctcctgct tctttgtcag cgcgtccacc agcccgtggg tcgcggcgat
     9841 cctcccacat gtcatcagca ggatgggcaa gctggagcag gtggacgtga accttttctg
     9901 cctggtcgcc acagacttct acagacacca gatagaggag gagctcgacc gcagggcctt
     9961 ccagtctgtg cttgaggtgg ttgcagcccc aggaagccca tatcaccggc tgctgacttg
    10021 tttacgaaat gtccacaagg tcaccacctg ctaaaacctc tacttccaat cgcaccatca
    10081 tcaccatcac catcaccacc atgattacaa ggatgacgac gataagtgag gcggccgcca
    10141 acttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    10201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    10261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    10321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    10381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    10441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    10501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    10561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    10621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcgggc gcccccctcc
    10681 tcacggcgag cgaagcttgt cgagaagtac tagaggatct ctagagcctg cagtctcgac
    10741 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
    10801 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    10861 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10981 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat
    11041 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
    11101 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
    11161 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
    11221 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
    11281 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
    11341 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
    11401 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    11461 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    11521 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    11581 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    11641 ttttcgccct ttgacgttgg agtccacgtt cttaatagtg gactcttgtt ccaaactgga
    11701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    11761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    11821 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    11881 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    11941 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    12001 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    12061 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    12121 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    12181 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    12241 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    12301 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    12361 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    12421 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    12481 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    12541 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    12601 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    12661 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    12721 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    12781 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    12841 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    12901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    12961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    13021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    13081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    13141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    13201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    13261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    13321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    13381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    13441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    13501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    13561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    13621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    13681 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    13741 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
    13801 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
    13861 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
    13921 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
    13981 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
    14041 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
    14101 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
    14161 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
    14221 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
    14281 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttcaaga gattgatgag
    14341 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
    14401 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
    14461 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
    14521 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
    14581 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
    14641 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
    14701 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
    14761 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
    14821 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
    14881 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
    14941 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
    15001 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
    15061 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
    15121 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
    15181 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
    15241 atagttctag tggttggcta cgtacccgta gtggctatgg cagggcttgc cgccccgacg
    15301 ttggctgcga gccctgggcc ttcacccgaa cttgggggtt ggggtgggga aaaggaagaa
    15361 acgcgggcgt attggtccca atggggtctc ggtggggtat cgacagagtg ccagccctgg
    15421 gaccgaaccc cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt
    15481 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
    15541 cccccatctc ccggtaccgc atg
//
