LOCUS       sequence-247727-        4268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression vector for Escherichia coli. BglBrick 
            compatible..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Jervis AJ, Carbonell P, Taylor S, Sung R, Dunstan MS, Robinson CJ, 
            Breitling R, Takano E, Scrutton NS
  TITLE     SelProm: A Queryable and Predictive Expression Vector Selection Tool
            for Escherichia coli.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399.
  PUBMED    30870592
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00399."
COMMENT     Sequence Label: pBbS9a-RFP
FEATURES             Location/Qualifiers
     source          1..4268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..42
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
     CDS             88..765
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      798..869
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      885..912
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1443..2393)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3155..3249)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3279..4070,4071..4139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3902..3921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4140..4244)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgtcctg atggctagct cagtcctagg gattatgcta gcaggtttaa tcgaattcaa
       61 aagatctttt aagaaggaga tatacatatg gcgagtagcg aagacgttat caaagagttc
      121 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      181 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      241 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      301 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      361 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      421 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      481 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
      541 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
      601 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
      661 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
      721 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaaggatc caaactcgag
      781 taaggatctc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatacc tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg
      961 tgttgctagt ttgttatcag aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc
     1021 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     1081 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     1141 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     1201 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     1261 catggtgaac agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     1321 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatgtaa cggtgaacag
     1381 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     1441 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     1501 agccatgaga acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc
     1561 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     1621 ttatgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     1681 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     1741 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     1801 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     1861 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     1921 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     1981 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     2041 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     2101 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     2161 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     2221 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     2281 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     2341 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     2401 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     2461 ctagtccttt tcccgggtga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     2521 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     2581 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     2641 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     2701 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     2761 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     2821 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     2881 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     2941 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     3001 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     3061 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     3121 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     3181 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3241 acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4261 gtgccacc
//
