LOCUS       sequence-247835-        4744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminal cysteine Abeta(1-42).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Zhang S, Guaglianone G, Morris MA, Yoo S, Howitz WJ, Xing L, Zheng 
            JG, Jusuf H, Huizar G, Lin J, Kreutzer AG, Nowick JS
  TITLE     Expression of N-Terminal Cysteine Abeta(42) and Conjugation to 
            Generate Fluorescent and Biotinylated Abeta(42).
  JOURNAL   Biochemistry. 2021 Apr 20;60(15):1191-1200. doi: 
            10.1021/acs.biochem.1c00105. Epub 2021 Apr 1.
  PUBMED    33793198
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.1c00105"; journalName: "Biochemistry"; date: 
            "2021-04-20- 20"; volume: "60"; issue: "15"; pages: "1191-1200"
COMMENT     Sequence Label: pET-Sac-Abeta(MC1-42)
FEATURES             Location/Qualifiers
     source          1..4744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(274..292)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      288..335
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(701..706,707..723,724..729))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(756..774)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        842..946
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(947..1015,1016..1807)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1165..1184)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1978..2566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2467..2486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2720..2737
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2752..2891
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2817..2836)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2977..2999
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2993..3184)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4642..4661
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atgtgcgacg ctgaattccg tcacgactct ggttacgaag
      121 ttcaccacca gaagctggtg ttcttcgctg aagacgtggg ttctaacaag ggtgctatca
      181 tcggtctgat ggttggtggc gttgtgatcg cttaatagga gctcgatccg gctgctaaca
      241 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      301 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      361 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
      421 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
      481 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
      541 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
      601 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
      661 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata
      721 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      781 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      841 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      901 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      961 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1021 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1081 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1141 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     1201 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1261 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1321 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1381 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1441 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     1501 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1561 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1621 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1681 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1741 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1801 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2101 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     2161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2581 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2641 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2701 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2761 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca
     2821 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     2881 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2941 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     3001 tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt
     3061 gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa
     3121 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg
     3181 tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac
     3241 gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt
     3301 gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc
     3361 aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg
     3421 cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg
     3481 aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc
     3541 agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc
     3601 gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc
     3661 caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     3721 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     3781 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     3841 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     3901 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     3961 tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     4021 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     4081 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     4141 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     4201 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     4261 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     4321 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     4381 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     4441 cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     4501 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     4561 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     4621 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     4681 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     4741 aaat
//
