LOCUS       sequence-247842-        2468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Prrn (N. tabacum) BPrrn20
FEATURES             Location/Qualifiers
     source          1..2468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(674..691)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(784..1372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(864..883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1546..2340)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2341..2445)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caggaggctc ccccgccgtc gttcaatgag
      421 aatggataag aggctcgtgg gattgacgtg agggggcagg gatggctatt gttctgggag
      481 cgaactccgg gcgaatacga agcgcttgga tacagttgta gggagggatt ttacttgaga
      541 ccggtacctg gagcacaaga ctggcctcat gggccttcct ttcactgccc gctttccagt
      601 cgggaaacct gtcgtgccag ctgcattaac atggtcatag ctgtttcctt gcgtattggg
      661 cgctctccgc ttcctcgctc actgactcgc tgcgctcggt cgttcgggta aagcctgggg
      721 tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttattaga aaaattcatc
     1561 cagcagacga taaaacgcaa tacgctggct atccggtgcc gcaatgccat acagcaccag
     1621 aaaacgatcc gcccattcgc cgcccagttc ttccgcaata tcacgggtgg ccagcgcaat
     1681 atcctgataa cgatccgcca cgcccagacg gccgcaatca ataaagccgc taaaacggcc
     1741 attttccacc ataatgttcg gcaggcacgc atcaccatgg gtcaccacca gatcttcgcc
     1801 atccggcatg ctcgctttca gacgcgcaaa cagctctgcc ggtgccaggc cctgatgttc
     1861 ttcatccaga tcatcctgat ccaccaggcc cgcttccata cgggtacgcg cacgttcaat
     1921 acgatgtttc gcctgatgat caaacggaca ggtcgccggg tccagggtat gcagacgacg
     1981 catggcatcc gccataatgc tcactttttc tgccggcgcc agatggctag acagcagatc
     2041 ctgacccggc acttcgccca gcagcagcca atcacggccc gcttcggtca ccacatccag
     2101 caccgccgca cacggaacac cggtggtggc cagccagctc agacgcgccg cttcatcctg
     2161 cagctcgttc agcgcaccgc tcagatcggt tttcacaaac agcaccggac gaccctgcgc
     2221 gctcagacga aacaccgccg catcagagca gccaatggtc tgctgcgccc aatcatagcc
     2281 aaacagacgt tccacccacg ctgccgggct acccgcatgc aggccatcct gttcaatcat
     2341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2461 agtgccac
//
