LOCUS       sequence-247845-        2505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Zm PclpP::Gg10 leader
FEATURES             Location/Qualifiers
     source          1..2505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(711..728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(821..1409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(901..920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1583..2377)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2378..2482)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag gcgcgccagg tctcaggaga aagagctcgg atcctctatg
      421 tattaataga atctatagta ttcttataga ataagaaaaa aaaaatgaag ataataaact
      481 gcggattctt tctttctctt ccattcttac gtttccatat taaagtgtag tttttttact
      541 taaatttaaa gaaggagata tacccaatgt gagaccatta attaatggag cacaagactg
      601 gcctcatggg ccttcctttc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      661 cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact
      721 gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc
      781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1261 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1501 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1561 agtaaacttg gtctgacagt tattagaaaa attcatccag cagacgataa aacgcaatac
     1621 gctggctatc cggtgccgca atgccataca gcaccagaaa acgatccgcc cattcgccgc
     1681 ccagttcttc cgcaatatca cgggtggcca gcgcaatatc ctgataacga tccgccacgc
     1741 ccagacggcc gcaatcaata aagccgctaa aacggccatt ttccaccata atgttcggca
     1801 ggcacgcatc accatgggtc accaccagat cttcgccatc cggcatgctc gctttcagac
     1861 gcgcaaacag ctctgccggt gccaggccct gatgttcttc atccagatca tcctgatcca
     1921 ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg atgtttcgcc tgatgatcaa
     1981 acggacaggt cgccgggtcc agggtatgca gacgacgcat ggcatccgcc ataatgctca
     2041 ctttttctgc cggcgccaga tggctagaca gcagatcctg acccggcact tcgcccagca
     2101 gcagccaatc acggcccgct tcggtcacca catccagcac cgccgcacac ggaacaccgg
     2161 tggtggccag ccagctcaga cgcgccgctt catcctgcag ctcgttcagc gcaccgctca
     2221 gatcggtttt cacaaacagc accggacgac cctgcgcgct cagacgaaac accgccgcat
     2281 cagagcagcc aatggtctgc tgcgcccaat catagccaaa cagacgttcc acccacgctg
     2341 ccgggctacc cgcatgcagg ccatcctgtt caatcatact cttccttttt caatattatt
     2401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
