LOCUS       sequence-247846-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-PaccD::5'UTR
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(808..825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(918..1506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(998..1017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1680..2474)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2475..2579)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatg aagggcgcgc caggtctcag gagaaaagag ctccgacata ggagaagccg
      421 ccctttatta aaaattatat tattttaaat aatataaagg gggttccaac atattaatat
      481 atagtgaagt gttcccccag attcagaact ttttttcaat actcacaatc cttattagtt
      541 aataatccta gtgattggat ttctatgctt agtctgatag gaaataagat attcaaataa
      601 ataattttat agcgaatgac tattcatcta ttgtattttc atgcaaatag ggggcaagaa
      661 aactctatga atgtgagacc attaattaac aacctgggcc tcatgggcct tcctttcact
      721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc atagctgttt
      781 ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      841 ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
     1681 tagaaaaatt catccagcag acgataaaac gcaatacgct ggctatccgg tgccgcaatg
     1741 ccatacagca ccagaaaacg atccgcccat tcgccgccca gttcttccgc aatatcacgg
     1801 gtggccagcg caatatcctg ataacgatcc gccacgccca gacggccgca atcaataaag
     1861 ccgctaaaac ggccattttc caccataatg ttcggcaggc acgcatcacc atgggtcacc
     1921 accagatctt cgccatccgg catgctcgct ttcagacgcg caaacagctc tgccggtgcc
     1981 aggccctgat gttcttcatc cagatcatcc tgatccacca ggcccgcttc catacgggta
     2041 cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg gacaggtcgc cgggtccagg
     2101 gtatgcagac gacgcatggc atccgccata atgctcactt tttctgccgg cgccagatgg
     2161 ctagacagca gatcctgacc cggcacttcg cccagcagca gccaatcacg gcccgcttcg
     2221 gtcaccacat ccagcaccgc cgcacacgga acaccggtgg tggccagcca gctcagacgc
     2281 gccgcttcat cctgcagctc gttcagcgca ccgctcagat cggttttcac aaacagcacc
     2341 ggacgaccct gcgcgctcag acgaaacacc gccgcatcag agcagccaat ggtctgctgc
     2401 gcccaatcat agccaaacag acgttccacc cacgctgccg ggctacccgc atgcaggcca
     2461 tcctgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2581 acatttcccc gaaaagtgcc ac
//
