LOCUS       sequence-24785-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L
  TITLE     Improved efficiency and pace of generating induced pluripotent stem 
            cells from human adult and fetal fibroblasts.
  JOURNAL   Stem Cells. 2008 Aug . 26(8):1998-2005.
  PUBMED    18511599
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2008 Aug . 26(8):1998-2005."
COMMENT     Sequence Label: Ef1a_LIN28_Ires_Puro
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attntnnttt agnnnngtga ggattagctt ggtactaata cgactcacta tagggagacc
       61 caagctggct aggtaagctt gatatcgaat tcgcccttat cgatgggctc cgtgtccaac
      121 cagcagtttg caggtggctg cgccaaggcg gcagaagagg cgcccgagga ggcgccggag
      181 gacgcggccc gggcggcgga cgagcctcag ctgctgcacg gtgcgggcat ctgtaagtgg
      241 ttcaacgtgc gcatggggtt cggcttcctg tccatgaccg cccgcgccgg ggtcgcgctc
      301 gaccccccag tggatgtctt tgtgcaccag agtaagctgc acatggaagg gttccggagc
      361 ttgaaggagg gtgaggcagt ggagttcacc tttaagaagt cagccaaggg tctggaatcc
      421 atccgtgtca ccggacctgg tggagtattc tgtattggga gtgagaggcg gccaaaagga
      481 aagagcatgc agaagcgcan atcaaaagga gacaggtgct acaactgtgg aggtctagat
      541 catcatgcca aggaatgcaa gctgccaccc cagcccaaga agtgccactt ctgccagagc
      601 atcanccata tggtagcctc atgtccgctg aaggcccagc agggccctan tgcacaggga
      661 aagccaacct actttcnaga ggaagaanaa naaatccaca gccctaccct gctcccggan
      721 gcacanaatt gacganaagg cnaattccgc cccc
//
