LOCUS       sequence-24786-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L
  TITLE     Improved efficiency and pace of generating induced pluripotent stem 
            cells from human adult and fetal fibroblasts.
  JOURNAL   Stem Cells. 2008 Aug . 26(8):1998-2005.
  PUBMED    18511599
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2008 Aug . 26(8):1998-2005."
COMMENT     Sequence Label: Ef1a_Large T-antigen_Ires_Puro
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             462..482
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attagcttgg tactaatacg actcactata gggagaccca agctggctag gtaagcttga
       61 tatcgaattc gcccttatcg tctagaatgg ataaagtttt aaacagagag gaatctttgc
      121 agctaatgga ccttctaggt cttgaaagga gtgcctgggg gaatattcct ctgatgagaa
      181 aggcatattt aaaaaaatgc aaggagtttc atcctgataa aggaggagat gaagaaaaaa
      241 tgaagaaaat gaatactctg tacaagaaaa tggaagatgg agtaaaatat gctcatcaac
      301 ctgactttgg aggcttctgg gatgcaactg agattccaac ctatggaact gatgaatggg
      361 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
      421 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
      481 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
      541 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
      601 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
      661 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aata
//
