LOCUS       sequence-247861-        9188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  clone BE4max-NG CBEs by SapI Golden Gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson 
            C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
  TITLE     Continuous evolution of base editors with expanded target 
            compatibility and improved activity.
  JOURNAL   Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0.
  PUBMED    31332326
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0."
COMMENT     Sequence Label: pBT362
FEATURES             Location/Qualifiers
     source          1..9188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3797..4045
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4076..4324
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4367..4387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4396..4413
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4436..4453)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4442..4666
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4737..4753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4737..4753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4750..4772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4761..4777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4785..4791,4792..4809,4810..4815))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4830..4851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4968..4985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5219..5238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5898..6689,6690..6758))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6521..6540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6759..6863)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6942..6961)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7133..7512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7513..7716
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7667..7687
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7758..7777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7758..7776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7826..7846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(7864..7891)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(7907..7978)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(7996..8673)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             8680..8691
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    8709..8727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8734..8752
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(8854..9188,1..3766)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
       61 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
      121 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
      181 cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa
      241 ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc
      301 tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa
      361 gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa
      421 gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga
      481 cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc
      541 caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac
      601 caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac
      661 cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga
      721 ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta
      781 caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct
      841 gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca
      901 gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct
      961 gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg
     1021 ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat
     1081 caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga
     1141 gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct
     1201 gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg
     1261 aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt
     1321 caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga
     1381 gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac
     1441 ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga
     1501 ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga
     1561 ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg
     1621 gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa
     1681 gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt
     1741 catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt
     1801 gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat
     1861 taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg
     1921 gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg
     1981 acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag
     2041 ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct
     2101 gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct
     2161 gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga
     2221 caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga
     2281 agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac
     2341 ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa
     2401 ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca
     2461 gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt
     2521 gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta
     2581 caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt
     2641 gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta
     2701 caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac
     2761 cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc
     2821 caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt
     2881 gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa
     2941 tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta tccggcccaa
     3001 gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg
     3061 cttcgtgagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa
     3121 gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag
     3181 cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga
     3241 cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat
     3301 gctggcctct gcccggttcc tgcagaaggg aaacgaactg gccctgccct ccaaatatgt
     3361 gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga
     3421 gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat
     3481 cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc
     3541 ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt
     3601 taccctgacc aatctgggag cccctcgggc cttcaagtac tttgacacca ccatcgaccg
     3661 gaaggtgtac cggagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac
     3721 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggtgacagcg gcgggagcgg
     3781 cgggagcggg gggagcacta atctgagcga catcattgag aaggagactg ggaaacagct
     3841 ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg gaggaagtga tcggcaacaa
     3901 gccagagtct gacatcctgg tgcacaccgc ctacgacgag tccacagatg agaatgtgat
     3961 gctgctgacc tctgacgccc ccgagtataa gccttgggcc ctggtcatcc aggattctaa
     4021 cggcgagaat aagatcaaga tgctgagcgg aggatccgga ggatctggag gcagcaccaa
     4081 cctgtctgac atcatcgaga aggagacagg caagcagctg gtcatccagg agagcatcct
     4141 gatgctgccc gaagaagtcg aagaagtgat cggaaacaag cctgagagcg atatcctggt
     4201 ccataccgcc tacgacgaga gtaccgacga aaatgtgatg ctgctgacat ccgacgcccc
     4261 agagtataag ccctgggctc tggtcatcca ggattccaac ggagagaaca aaatcaaaat
     4321 gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag
     4381 gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     4441 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4501 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4561 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4621 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4681 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     4741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4801 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgcactt ccgcttcctc gctcactgac
     4981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5761 actcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5821 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5881 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5941 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6001 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
     6061 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6121 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6181 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6241 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6301 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6361 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6421 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6481 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6541 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6601 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6661 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6721 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6781 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6841 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     6901 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     6961 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     7021 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     7081 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     7141 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     7201 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     7261 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     7321 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     7381 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     7441 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     7501 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     7561 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     7621 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     7681 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
     7741 tagagatccg cggccgctaa tacgactcac tatagggaga gccgccacca tgaaacggac
     7801 agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcagta gaagagctat
     7861 aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg
     7921 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     7981 gcctggagat ccttattaag caccggtgga gtgacgacct tcagcacgtt cgtactgttc
     8041 aacgatggtg tagtcttcgt tgtgggaggt gatgtccagt ttgatgtcgg ttttgtaagc
     8101 acccggcagc tgaaccggtt ttttagccat gtaggtggtt ttaacttcag cgtcgtagtg
     8161 accaccgtct ttcagtttca gacgcatttt gatttcacct ttcagagcac cgtcttccgg
     8221 gtacatacgt tcggtggaag cttcccaacc catggttttt ttctgcataa ccggaccgtc
     8281 ggacgggaag ttggtaccac gcagtttaac tttgtagatg aactcaccgt cttgcaggga
     8341 ggagtcctgg gtaacggtaa caacaccacc gtcttcgaag ttcataacac gttcccattt
     8401 gaaaccttcc gggaaggaca gtttcaggta gtccgggatg tcagccgggt gtttaacgta
     8461 agctttggaa ccgtactgga actgcgggga caggatgtcc caagcgaacg gcagcggacc
     8521 acctttggta actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc
     8581 accttcgatt tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat
     8641 gaactctttg ataacgtctt cgctactcgc catagatcct ttctcctctt tcagatccgt
     8701 gctcagtatc tctatcactg atagggatgt caatctctat cactgatagg gagctcttct
     8761 ggcggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca
     8821 ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc
     8881 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     8941 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     9001 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
     9061 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     9121 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     9181 aagaagca
//
