LOCUS       sequence-247864-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing BenR transcription factor gene under control of benzoate 
            responsive promoter (pBen) in a feedback loop..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pSB4C5_pBen-BenR
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             677..1336
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(743..762)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2562..2633
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2713..2807
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3299..3521
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(3569..4158,1..361)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tttaaacact tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac
       61 gttgattttc caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa
      121 ccagataaaa atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctacg
      181 taacggacta agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt
      241 tgaggcaaaa tttttgagtg acatgcaaag taagcatgat ctcaatggtt cgttctcatg
      301 gctcacgcaa aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg
      361 aggttcttat ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa
      421 caactgttca ccgttagata tcaaagggaa aactgtccat atgcacagat gaaaacggtg
      481 taaaaaagat agatacatca gagcttttac gagtttttgg tgcatttaaa gctgttcacc
      541 atgaacagat cgacaatgta actactagag gttgatcggg cacgtaagag gttccaactt
      601 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      661 agctaaggaa gctaaaatgg agaaaaaaat cacgggatat accaccgttg atatatccca
      721 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      781 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      841 ttatccggcc tttattcaca ttcttgcccg cctgatgaac gctcacccgg agtttcgtat
      901 ggccatgaaa gacggtgagc tggtgatctg ggatagtgtt cacccttgtt acaccgtttt
      961 ccatgagcaa actgaaacgt tttcgtccct ctggagtgaa taccacgacg atttccggca
     1021 gtttctccac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1081 taaagggttt attgagaata tgttttttgt ctcagccaat ccctgggtga gtttcaccag
     1141 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca cgatgggcaa
     1201 atattatacg caaggcgaca aggtgctgat gccgctggcg atccaggttc atcatgccgt
     1261 ttgtgatggc ttccatgtcg gccgcatgct taatgaatta caacagtact gtgatgagtg
     1321 gcagggcggg gcgtaataat actagctccg gcaaaaaaac gggcaaggtg tcaccaccct
     1381 gccctttttc tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg tctaagacga
     1441 tactgttcga agcattgcca ttttctgaag ttaccgaaaa agtaccgaac atccgtaaat
     1501 ctggataacg ttctgcacaa tccggatagc cccccgccag ccgtctccct aacctgacca
     1561 ggtctaaaca ataacaaggg agagtctggc catggaatct cgtctgctgt ctgaacgttc
     1621 ttctgttttc caccacgctg acccgtacgc tgtttctgac tacgttaacc agcacgttgg
     1681 tcagcactgc atcggtctgt ctcgtaccac ccacccgcag gcttctctgt ctcaccgtaa
     1741 attcgctgaa ctggacctgt gccgtatctc ttacggtggt tctgttcgtg ttacctctcc
     1801 ggctctggaa accatctacc acctgcaggt tctgctgaac ggtaactgcc tgtggcgtgg
     1861 tcacaaacgt gaacagcacc tggttccggg tgaactgctg ctgatcaacc cggacgaccc
     1921 ggttgacctg acctactctg aagactgcga aaaattcatc ctgaaagttc cgacccgtct
     1981 gctggactct atctgcgacg aacagcgttg gcagcgtccg gacggtggtg ttcgtttcct
     2041 gcgtaaccac taccgtctgg acgaactgga cggtttcgtt aacctgctgg ctatggtttg
     2101 ccacgaagct gaagtttctg actctctgcc gcgtgttcag ggtcactact ctcagatcgt
     2161 tgcttctaaa ctgctgaccc tgatgtctac caacatccgt cgtgaatctc tgtctgctcc
     2221 gcaggctggt ctggaacgta tcctggacta catcgaacgt aacctgaaac tggaactgtc
     2281 tgctgaagtt ctggctgaac aggcttgcat gtctctgcgt tctctgtacg ctctgttcga
     2341 ccagcacctg ggtatcaccc cgaaacacta cgttcgtcag cgtaaactgg aacgtgttca
     2401 cgcttgcctg tctgacccga cctgcggtgt tcgttctgtt accgaactgg ctctggacta
     2461 cggtttcctg cacctgggtc gtttctctga aatctaccgt cagcagttcg gtgaactgcc
     2521 gtctcagacc ttcaaacgtc gtgcttaata gacccaggca tcaaataaaa cgaaaggctc
     2581 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2641 gtcacactgg ctcaccttcg ggtgggccga ctttctgcta gagctgtcag accaagttta
     2701 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     2761 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcactaggg
     2821 acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc
     2881 tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac
     2941 tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag
     3001 aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg
     3061 cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg
     3121 tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga
     3181 tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag
     3241 aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact aaagtagtga
     3301 gttatacaca gggctgggat ctattctttt tatctttttt tattctttct ttattctata
     3361 aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga
     3421 gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac
     3481 aactcaaagg aaaaggacat gtaattatca ttgactagcc catctcaatt ggtatagtga
     3541 ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga
     3601 actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt
     3661 ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac
     3721 ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc
     3781 taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt tggttaaagg
     3841 ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt
     3901 ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct
     3961 ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt ggttattaaa
     4021 agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa
     4081 gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt
     4141 gaaaccaata agtaaaga
//
