LOCUS       sequence-24787-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K
  TITLE     Characterization and subcellular targeting of GCaMP-type 
            genetically-encoded calcium indicators.
  JOURNAL   PLoS ONE. 2008  . 3(3):e1796.
  PUBMED    18350138
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(3):e1796."
COMMENT     Sequence Label: CMV-GCaMP2
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             88..105
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             110..142
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(146..154,155..169)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             197..253
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     466..487
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(593..614)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggtgggnnn ttagcagagc tggtttgttg aaccgtcaga tccgctagcg ctaccggact
       61 cagatctcgc caccatgcgg ggnttctcat catcatcatc atcatggnta tggctagcat
      121 gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg atctcgccac
      181 catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca gagctatagg
      241 tcggctgagc tcactcgaga acgtctatat catggccgac aagcagaaga acggcatcaa
      301 ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta
      361 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      421 cacccagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      481 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg
      541 agggagcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      601 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc
      661 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc nggtgccctg
      721 gcccaccctc gtgacacctg acttacgcgt gnagtgctnc agccgctacc cgacactgag
      781 cagcagactc tcagtcgcnt gccgagntat ncngacgcnc tnttttagag aggnananaa
      841 acncccgnga gtatttgngn cacctgtgac
//
