LOCUS       sequence-247878-        2511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pOK-mPrrn::RBS (20%)
FEATURES             Location/Qualifiers
     source          1..2511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     393..410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     394..410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     421..440
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        421..439
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(628..639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(731..748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      785..828
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(885..1473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(965..984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1647..2441)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2442..2505)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctaataaca ggcctgctgg taatcgcagg cctttttatt tggtgtattt agaaaaataa
       61 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
      121 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      181 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtggccgc
      241 tacagggcgc tcccattcgc cattcaggct gcgcaactgt tgggaagggc gtttcggtgc
      301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgacg
      421 taatacgact cactataggg cgaattgtag aaggaaggcc gtcaaggcct aggcgcgcca
      481 tgagctcggt ctcaggagtt gacgtgaggg ggcagggatg gcaatatatc tgggagcgaa
      541 ctccgggcga atacgaagcg cttggataca gttgtaggga gggatttaat gtgagaccgg
      601 tacctcttaa ttaactggcc tcatgggcct tccttctatc actgcccgct ttccagtcgg
      661 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc
      721 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc
      781 ctaaataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattg agcaaaaggc
      841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1621 tatgagtaaa cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca
     1681 atacgctggc tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg
     1741 ccgcccagtt cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc
     1801 acgcccagac ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc
     1861 ggcaggcacg catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc
     1921 agacgcgcaa acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga
     1981 tccaccaggc ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga
     2041 tcaaacggac aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg
     2101 ctcacttttt ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc
     2161 agcagcagcc aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca
     2221 ccggtggtgg ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg
     2281 ctcagatcgg ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc
     2341 gcatcagagc agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac
     2401 gctgccgggc tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat
     2461 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga a
//
