LOCUS       sequence-24788-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K
  TITLE     Characterization and subcellular targeting of GCaMP-type 
            genetically-encoded calcium indicators.
  JOURNAL   PLoS ONE. 2008  . 3(3):e1796.
  PUBMED    18350138
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(3):e1796."
COMMENT     Sequence Label: pCAGGS-GCaMP2-actin
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             141..173
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(177..185,186..200)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      207..216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             228..284
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     497..518
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(624..645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctacatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
       61 ctgtctcatc attttggcaa agaattcggt accgcgggcc cgccaccatg cggggttctc
      121 atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc
      181 tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt cgtaagtgga
      241 ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag aacgtctata
      301 tcatggccga caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg
      361 aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc ggcgacggcc
      421 ccgtgctgct gcccgacaac cactacctga gcacccagtc caaactttcg aaagacccca
      481 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      541 gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc
      601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      661 tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc ctganntcat
      721 ctgcaccgac
//
