LOCUS       sequence-247880-        2464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-mPrrn::RBS (60%)
FEATURES             Location/Qualifiers
     source          1..2464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(670..687)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(780..1368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(860..879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1542..2336)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2337..2441)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggcctaggc gcgccatgag ctcggtctca ggaggctccc ccgccgtcgt tcaatgagaa
      421 tggataagag gctcgtggga ttgacgtgag ggggcaggga tggcataatt tctgggagcg
      481 aactccgggc gaatacgaag cgcttggata cagttgtagg gagggattta atgtgagacc
      541 ggtacctctt aattaactgg cctcatgggc cttcctttca ctgcccgctt tccagtcggg
      601 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct
      661 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc
      721 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      781 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      841 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      901 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      961 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1021 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1081 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1141 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1201 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1261 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1321 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1381 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1441 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1501 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa ttcatccagc
     1561 agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag caccagaaaa
     1621 cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag cgcaatatcc
     1681 tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa acggccattt
     1741 tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc ttcgccatcc
     1801 ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg atgttcttca
     1861 tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg ttcaatacga
     1921 tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag acgacgcatg
     1981 gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag cagatcctga
     2041 cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac atccagcacc
     2101 gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc atcctgcagc
     2161 tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc ctgcgcgctc
     2221 agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc atagccaaac
     2281 agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc aatcatactc
     2341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2461 ccac
//
