LOCUS       sequence-247884-        2443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-Prrn::RBS
FEATURES             Location/Qualifiers
     source          1..2443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(649..666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(759..1347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(839..858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1521..2315)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2316..2420)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct caggagtaag aggctcgtgg gattgacgtg
      421 agggggcagg gatggctata tttctgggag cgaactccgg gcgaatatga agcgcatcga
      481 tacaagtgag ttgtagggag ggaaccaatg tgagaccggt acctggagca caagactggc
      541 ctcatgggcc ttcctttcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      601 ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct cgctcactga
      661 ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa
      721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1501 taaacttggt ctgacagtta ttagaaaaat tcatccagca gacgataaaa cgcaatacgc
     1561 tggctatccg gtgccgcaat gccatacagc accagaaaac gatccgccca ttcgccgccc
     1621 agttcttccg caatatcacg ggtggccagc gcaatatcct gataacgatc cgccacgccc
     1681 agacggccgc aatcaataaa gccgctaaaa cggccatttt ccaccataat gttcggcagg
     1741 cacgcatcac catgggtcac caccagatct tcgccatccg gcatgctcgc tttcagacgc
     1801 gcaaacagct ctgccggtgc caggccctga tgttcttcat ccagatcatc ctgatccacc
     1861 aggcccgctt ccatacgggt acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac
     1921 ggacaggtcg ccgggtccag ggtatgcaga cgacgcatgg catccgccat aatgctcact
     1981 ttttctgccg gcgccagatg gctagacagc agatcctgac ccggcacttc gcccagcagc
     2041 agccaatcac ggcccgcttc ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg
     2101 gtggccagcc agctcagacg cgccgcttca tcctgcagct cgttcagcgc accgctcaga
     2161 tcggttttca caaacagcac cggacgaccc tgcgcgctca gacgaaacac cgccgcatca
     2221 gagcagccaa tggtctgctg cgcccaatca tagccaaaca gacgttccac ccacgctgcc
     2281 gggctacccg catgcaggcc atcctgttca atcatactct tcctttttca atattattga
     2341 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2401 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
