LOCUS       sequence-247889-        9821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid of OptoTGFBRs 
            (optogenetically-activated TGFb receptors).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Li Y, Lee M, Kim N, Wu G, Deng D, Kim JM, Liu X, Heo WD, Zi Z
  TITLE     Spatiotemporal Control of TGF-beta Signaling with Light.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):443-451. doi: 
            10.1021/acssynbio.7b00225. Epub 2018 Jan 12.
  PUBMED    29241005
REFERENCE   2  (bases 1 to 9821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00225"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "443-451"
COMMENT     Sequence Label: pCMV-OptoTGFBRs
FEATURES             Location/Qualifiers
     source          1..9821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      535..544
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1714..2223
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             2245..2301
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4990..4999
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4996..6426
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    6549..6670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6586..6605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6640..6659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6677..7132)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6814..6835)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7026..7045
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7159..7263
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7265..7622
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7265..7456
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7288..7308)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7473..7608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7657..8451
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7657..8451
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7711..7730)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8321..8340
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8639..8658)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8683..8730
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9059..9647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9548..9567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
      541 atgggatgta taaaatcaaa agggaaagac agcgcgggag cagatagtgc tggtagtgct
      601 ggtagtgctg ggaattcgtg ccataaccgc actgtcattc accaccgtgt gccaaatgaa
      661 gaggatccat cactagatcg ccctttcatt tcagagggca ccaccttaaa agatttaatt
      721 tatgatatga caacatcagg gtctggatca ggtttaccac tgcttgttca aagaacaatt
      781 gccaggacca ttgtgttaca agaaagcatt ggcaaaggtc ggtttggaga agtttggcgg
      841 ggcaaatggc ggggagaaga agttgctgtg aagatattct cttctagaga agagcgttca
      901 tggttccgag aggcagagat ttatcagact gtaatgttac gccatgaaaa tatcctggga
      961 tttatagcag cagacaacaa agacaatggg acatggacgc agctgtggtt ggtgtcagat
     1021 tatcatgagc atggatccct tttcgattac ttgaatagat acactgttac tgtggaagga
     1081 atgatcaagc ttgctctgtc cacagcaagt ggtcttgccc atcttcacat ggagattgtt
     1141 ggtacccaag gaaaaccagc tattgcccat agagatttga aatcaaagaa tatcttggtg
     1201 aagaaaaatg gaacctgttg tattgcagac ttgggacttg ctgtgagaca tgattctgcc
     1261 acagatacaa ttgatattgc tccaaaccac agagtaggca ctaaaaggta catggcccct
     1321 gaagttctag atgattccat aaatatgaaa cattttgaat ccttcaaacg cgctgacatc
     1381 tatgcaatgg gcttagtgtt ctgggaaatt gctcgacgct gttctattgg tggaatccat
     1441 gaagactatc agttgcctta ttatgatctt gtaccttctg acccatcggt tgaagaaatg
     1501 agaaaagtag tttgcgaaca gaagttaagg ccaaatattc caaacagatg gcagagctgt
     1561 gaggccttga gagtgatggc taaaattatg agagaatgct ggtatgccaa tggagcagca
     1621 aggctgacag ctttgcgaat taaaaaaaca ttgtcacaac tcagccaaca ggaaggcatc
     1681 aaaatgggct cgagggatcc aagtgctggt ggcatgaatg gagctatagg aggtgacctt
     1741 ttgctcaatt ttcctgacat gtcggtccta gagcgccaaa gggctcacct caagtacctc
     1801 aatcccacct ttgattctcc tctcgccggc ttctttgccg attcttcaat gattaccggc
     1861 ggcgagatgg acagctatct ttcgactgcc ggtttgaatc ttccgatgat gtacggtgag
     1921 acgacggtgg aaggtgattc aagactctca atttcgccgg aaacgacgct tgggactgga
     1981 aatttcaagg cagcgaagtt tgatacagag actaaggatt gtaatgaggc ggcgaagaag
     2041 atgacgatga acagagatga cctagtagaa gaaggagaag aagagaagtc gaaaataaca
     2101 gagcaaaaca atgggagcac aaaaagcatc aagaagatga aacacaaagc caagaaagaa
     2161 gagaacaatt tctctaatga ttcatctaaa gtgacgaagg aattggagaa aacggattat
     2221 attcatgtac cggtgggctc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     2281 gtcgaagaga atcctggacc gggactagcc accatgggga attcgcgtgt ccaccggcag
     2341 cagaagctga gcccgtcctg ggagagcagc aagccccgga aactgatgga tttcagtgac
     2401 aattgtgcca tcatcctgga ggacgaccgc tccgacatca gctccacgtg cgccaacaac
     2461 atcaaccaca acacggagct gctgcccatc gagctggaca cgctggtggg gaagggccgc
     2521 ttcgccgagg tctacaaggc caagctgaag cagaacacct cagagcagtt tgagaccgtg
     2581 gctgtcaaga tcttccccta cgaggagtac tcctcgtgga aaacagagaa ggacatcttc
     2641 tccgatatca acctgaagca tgagaacatc ctgcagttcc tgacggccga ggagcggaag
     2701 acagagctgg gcaagcagta ctggctgatc acggcgttcc acgcgaaggg caacctgcag
     2761 gagtacctca cgaggcatgt catcagctgg gaggacctga ggaagctggg cagctccctg
     2821 gcccggggca tcgctcatct ccacagtgac cacactcctt gtgggaggcc caagatgccc
     2881 attgttcaca gggacctcaa gagctctaac atcctagtga agaacgactt gacctgttgc
     2941 ctgtgtgact tcgggctgtc cttgcgcctg gaccctactc tgtctgtgga tgacctggcc
     3001 aacagcgggc aggtgggaac ggcaagatac atggccccgg aagttctaga atccaggatg
     3061 aatctggaaa acgtggagtc gttcaagcag acggatgtct actccatggc tctggtactc
     3121 tgggaaatga cgtcccgctg caatgctgtg ggagaagtga aggattacga gcccccattt
     3181 ggttccaagg tgcgggagca cccctgtgtg gagagcatga aagacagtgt gctgagagac
     3241 cgagggcggc cggaaattcc cagcttctgg ctcaaccacc agggcatcca gatcgtgtgt
     3301 gagactttga ccgagtgctg ggaccatgac cccgaagccc gtctcacagc acagtgtgtg
     3361 gcagagcgct tcagtgagct ggagcatccg gagagactct ctgggaggag ctgctcccag
     3421 gagaagattc cagaagatgg ctcgctgaac actaccaaag gggatccgac aggtatgaag
     3481 atggacaaaa agaccatcgt ctggtttcgg agagatttga gaatagaaga taatcccgcg
     3541 ctcgccgccg cggcccacga gggttccgtc ttccccgttt tcatttggtg tcctgaagaa
     3601 gaaggccagt tttatcccgg aagggcctct aggtggtgga tgaagcaaag tctggcccat
     3661 cttagccagt cactgaaagc actgggcagt gatcttaccc tgatcaagac acacaatacc
     3721 atctctgcca tcctcgactg catcagggtg accggcgcaa cgaaagtcgt gtttaaccac
     3781 ctgtacgatc cagttagtct ggtgcgcgac cacactgtga aggagaagct ggtggaacgg
     3841 gggatcagtg tgcagagcta caacggggac cttctgtacg agccatggga gatctattgc
     3901 gagaaaggga aaccgttcac ctccttcaac agttactgga agaaatgttt ggatatgtca
     3961 atagagtccg ttatgttgcc ccctccctgg agactgatgc cgattactgc tgctgcagag
     4021 gccatctggg cctgctccat cgaggaactc ggtctggaaa atgaagcaga aaagccaagc
     4081 aatgcacttc tcactagagc ctggagcccc ggctggtcta atgccgacaa gctgcttaac
     4141 gagttcatcg aaaaacaact gattgactac gcgaagaact ccaagaaagt ggtaggtaac
     4201 tcaactagct tgctctctcc atatctccat tttggcgaga tttctgtccg ccatgtattt
     4261 cagtgcgctc ggatgaaaca gattatctgg gctcgcgata aaaacagcga aggcgaagaa
     4321 agcgccgatc tgttcctgcg agggatcgga cttcgggaat actcccggta tatatgtttc
     4381 aactttccat tcacacacga gcagagtctg ttgtcccacc tcaggttctt cccctgggac
     4441 gccgatgtcg acaaattcaa ggcatggaga cagggaagga cagggtaccc actcgtggat
     4501 gctggcatga gagagctctg ggctacaggc tggatgcaca accgcatccg ggtaatcgtg
     4561 tcctcatttg ctgtcaagtt tctgctcctg ccttggaaat ggggaatgaa gtacttttgg
     4621 gatacccttc tcgacgccga cttggagtgt gacattctgg gatggcaata tattagcggg
     4681 tcaattcctg acggccatga gttggacagg ttggacaatc cggccttgca gggagctaag
     4741 tatgatcccg aaggagagta tattcgacag tggctccccg agctggcccg acttcctacg
     4801 gagtggattc accatccttg ggacgcacca ctgacagtgc tcaaggcaag cggggtggag
     4861 ctgggcacca attacgctaa gcctatagtt gatatagata cagcacgcga gctgctggct
     4921 aaagcgatct ctcgcactcg ggaggcgcag attatgatcg gtgctgccgg cagcggcagc
     4981 ccaccggtcg ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
     5041 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     5101 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     5161 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     5221 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     5281 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     5341 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     5401 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     5461 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     5521 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     5581 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5641 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc
     5701 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg
     5761 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     5821 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     5881 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     5941 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     6001 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     6061 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     6121 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     6181 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     6241 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     6301 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     6361 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     6421 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     6481 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     6541 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6661 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     6721 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     6781 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6841 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6901 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6961 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7021 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     7081 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     7141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7261 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7441 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7561 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7621 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7681 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7741 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7801 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     7861 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7921 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7981 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     8041 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     8101 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8161 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     8221 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8281 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8341 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8401 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8461 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8521 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8581 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     8641 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     8701 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     8761 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     8821 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     8881 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     8941 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9001 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9061 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9121 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9181 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9241 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9301 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9361 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9421 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9481 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9541 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9601 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9661 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9721 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
     9781 attacggggt cattagttca tagcccatat atggagttcc g
//
