LOCUS       sequence-247894-        2363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-atpH 5'UTR processed -45
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(569..586)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(679..1267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(759..778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1441..2235)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2236..2340)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact attgtatcat taaccatttc ttttttttgg
      421 tacgaggaac ttatcaatgt gagaccggta ccctctgggc ctcatgggcc ttcctttcac
      481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt
      541 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      601 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa
      661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1441 ttagaaaaat tcatccagca gacgataaaa cgcaatacgc tggctatccg gtgccgcaat
     1501 gccatacagc accagaaaac gatccgccca ttcgccgccc agttcttccg caatatcacg
     1561 ggtggccagc gcaatatcct gataacgatc cgccacgccc agacggccgc aatcaataaa
     1621 gccgctaaaa cggccatttt ccaccataat gttcggcagg cacgcatcac catgggtcac
     1681 caccagatct tcgccatccg gcatgctcgc tttcagacgc gcaaacagct ctgccggtgc
     1741 caggccctga tgttcttcat ccagatcatc ctgatccacc aggcccgctt ccatacgggt
     1801 acgcgcacgt tcaatacgat gtttcgcctg atgatcaaac ggacaggtcg ccgggtccag
     1861 ggtatgcaga cgacgcatgg catccgccat aatgctcact ttttctgccg gcgccagatg
     1921 gctagacagc agatcctgac ccggcacttc gcccagcagc agccaatcac ggcccgcttc
     1981 ggtcaccaca tccagcaccg ccgcacacgg aacaccggtg gtggccagcc agctcagacg
     2041 cgccgcttca tcctgcagct cgttcagcgc accgctcaga tcggttttca caaacagcac
     2101 cggacgaccc tgcgcgctca gacgaaacac cgccgcatca gagcagccaa tggtctgctg
     2161 cgcccaatca tagccaaaca gacgttccac ccacgctgcc gggctacccg catgcaggcc
     2221 atcctgttca atcatactct tcctttttca atattattga agcatttatc agggttattg
     2281 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2341 cacatttccc cgaaaagtgc cac
//
