LOCUS       sequence-247896-        2542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-atpI 5'UTR
FEATURES             Location/Qualifiers
     source          1..2542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(748..765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(858..1446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(938..957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1620..2414)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2415..2519)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catactagat aaataactta cttcagaaat
      421 cccttatgga atcgcttact atttctgaat ttcaaaaaag agataaaaat agctggggat
      481 attatgtgat ttattagtat tctaaatctt agttggtatt caaaatatcc gattcaagta
      541 gacaaagaga tggttgaatc aaaaaatttt gtttaaagtt caattttttc agagggcaag
      601 gcaataatgt gagaccggta cctggagcac aagactggcc tcatgggcct tcctttcact
      661 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc atagctgttt
      721 ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      781 ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
     1621 tagaaaaatt catccagcag acgataaaac gcaatacgct ggctatccgg tgccgcaatg
     1681 ccatacagca ccagaaaacg atccgcccat tcgccgccca gttcttccgc aatatcacgg
     1741 gtggccagcg caatatcctg ataacgatcc gccacgccca gacggccgca atcaataaag
     1801 ccgctaaaac ggccattttc caccataatg ttcggcaggc acgcatcacc atgggtcacc
     1861 accagatctt cgccatccgg catgctcgct ttcagacgcg caaacagctc tgccggtgcc
     1921 aggccctgat gttcttcatc cagatcatcc tgatccacca ggcccgcttc catacgggta
     1981 cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg gacaggtcgc cgggtccagg
     2041 gtatgcagac gacgcatggc atccgccata atgctcactt tttctgccgg cgccagatgg
     2101 ctagacagca gatcctgacc cggcacttcg cccagcagca gccaatcacg gcccgcttcg
     2161 gtcaccacat ccagcaccgc cgcacacgga acaccggtgg tggccagcca gctcagacgc
     2221 gccgcttcat cctgcagctc gttcagcgca ccgctcagat cggttttcac aaacagcacc
     2281 ggacgaccct gcgcgctcag acgaaacacc gccgcatcag agcagccaat ggtctgctgc
     2341 gcccaatcat agccaaacag acgttccacc cacgctgccg ggctacccgc atgcaggcca
     2401 tcctgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2461 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2521 acatttcccc gaaaagtgcc ac
//
