LOCUS       sequence-247898-        8550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, 
            Shimizu Y, Suemori H, Nakatsuji N, Mitani K
  TITLE     Highly efficient transient gene expression and gene targeting in 
            primate embryonic stem cells with helper-dependent adenoviral 
            vectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13781-6.
  PUBMED    18768795
REFERENCE   2  (bases 1 to 8550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13781-6."
COMMENT     Sequence Label: pBRHDAd-TKVenus
FEATURES             Location/Qualifiers
     source          1..8550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   8..110
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     198..348
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     polyA_signal    1034..1082
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     1107..1126
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(1124..2254)
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMIQTHVTTPGSIPTICDLARTFAREMGEAN"
     5'UTR           2272..2311
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     promoter        complement(2333..2395)
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     primer_bind     2635..2651
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2651
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        2692..3676
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3711..3729
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3711..3728
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(3764..3766,3767..3769,3770..4483)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3809..3830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4070..4089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4534..4668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4558..4577)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4612..4631
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5082..5184
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        complement(5214..5232)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5253..5269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5253..5269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5266..5288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5277..5293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5301..5307,5308..5325,5326..5331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5346..5367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             5964..6155
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6149..6171)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6257..6397
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6312..6331
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6412..6429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6583..7171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6663..6682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7342..8133,8134..8202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7965..7984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8203..8307)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8375..8393
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     8468..8490
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8482..8499
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8483..8499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8509..8527
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtttaaacat catcaataat ataccttatt ttggattgaa gccaatatga taatgagggg
       61 gtggagtttg tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag tagtgtggcg
      121 gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac
      181 gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat
      241 gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat
      301 aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg
      361 ccgcggggac tttgaccgtt tacgtggaga ctcgcccagg tgtttttctc aggtgttttc
      421 cgcgttccgg gtcaaagttg gcgttttgat taaccctcac taaagggaac aaaagctgga
      481 gctccaccgc ggtggcttgc ggccgcaact agagcttgat ctggccgggg tcccgcggaa
      541 actcggccgt ggtgaccaat acaaaacaaa agcgctcctc gtaccagcga agaaggggca
      601 gagatgccgt agtcaggttt agttcgtccg gcggcgccag aaatccgcgc ggtggttttt
      661 gggggtcggg ggtgtttggc agccacagac gcccggtgtt cgtgtcgcgc cagtacatgc
      721 ggtccatgcc caggccatcc aaaaaccatg ggtctgtctg ctcagtccag tcgtggactg
      781 accccacgca acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga
      841 ccccgtccct aacccacggg gcccgtggct atggcagggc ctgccgcccc gacgttggct
      901 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg
      961 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga
     1021 accccgcgtt tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc
     1081 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca
     1141 tctcccgggc aaacgtgcgc gccaggtcgc agatcgtcgg tatggagcct ggggtggtga
     1201 cgtgggtctg gatcatcccg gaggtaagtt gcagcagggc gtcccggcag ccggcgggcg
     1261 attggtcgta atccaggata aagacgtgca tgggacggag gcgtttggcc aagacgtcca
     1321 aggcccaggc aaacacgttg tacaggtcgc cgttgggggc cagcaactcg ggggcccgaa
     1381 acagggtaaa taacgtgtcc ccgatatggg gtcgtgggcc cgcgttgctc tggggctcgg
     1441 caccctgggg cggcacggcc gtccccgaaa gctgtcccca atcctcccgc cacgacccgc
     1501 cgccctgcag ataccgcacc gtattggcaa gcagcccgta aacgcggcga atcgcggcca
     1561 gcatagccag gtcaagccgc tcgccggggc gctggcgttt ggccaggcgg tcgatgtgtc
     1621 tgtcctccgg aagggccccc aacacgatgt ttgtgccggg caaggtcggc gggatgaggg
     1681 ccacgaacgc cagcacggcc tggggggtca tgctgcccat aaggtatcgc gcggccgggt
     1741 agcacaggag ggcggcgatg ggatggcggt cgaagatgag ggtgagggcc gggggcgggg
     1801 catgtgagct cccagcctcc cccccgatat gaggagccag aacggcgtcg gtcacggcat
     1861 aaggcatgcc cattgttatc tgggcgcttg tcattaccac cgccgcgtcc ccggccgata
     1921 tctcaccctg gtcaaggcgg tgttgtgtgg tgtagatgtt cgcgattgtc tcggaagccc
     1981 ccagcacccg ccagtaagtc atcggctcgg gtacgtagac gatatcgtcg cgcgaaccca
     2041 gggccaccag cagttgcgtg gtggtggttt tccccatccc gtggggaccg tctatataaa
     2101 cccgcagtag cgtgggcatt ttctgctccg ggcggacttc cgtggcttct tgctgccggc
     2161 gagggcgcaa cgccgtacgt cggttgctat ggccgcgaga acgcgcagcc tggtcgaacg
     2221 cagacgcgtg ctgatggccg gggtacgaag ccatacgcgc ttctacaagg cgctggccga
     2281 agaggtgcgg gagtttcacg ccaccaagat ctgcggcacg ctgttgacgc tgttaagcgg
     2341 gtcgctgcag ggtcgctcgg tgttcgaggc cacacgcgtc accttaatat gcgaagtgga
     2401 cctgggaccg cgccgccccg actgcatctg cgtgttcgaa ttcgccaatg acaagacgct
     2461 gggcggggtt tgctcgacat tgggtggaaa cattccaggc ctgggtggag aggctttttg
     2521 cttcctcttg caaaaccaca ctgctcgaca ttgggtggaa acattccagg cctgggtgga
     2581 gaggcttttt gcttcctctt gaaaaccaca ctgctcgact ctagttgcgg ccgctctaga
     2641 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgaccgc
     2701 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
     2761 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
     2821 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
     2881 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
     2941 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
     3001 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
     3061 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
     3121 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
     3181 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
     3241 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
     3301 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
     3361 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
     3421 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
     3481 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
     3541 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
     3601 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     3661 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
     3721 cactatagaa tacaagctac ttgttctttt tgcaggatcc accatggtga gcaagggcga
     3781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3901 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     3961 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4201 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4261 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     4321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4441 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattca aggcctctcg
     4501 agcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
     4561 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4621 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4681 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
     4741 gcggcgccaa tgcattgggc ccgagcttgc atgcctgcag gttttaccag taaaaaagaa
     4801 aacctattaa aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa
     4861 gggccaagtg cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac
     4921 aaaaaacacc cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca
     4981 caacttcctc aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa
     5041 actacaattc ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt
     5101 cccacgcccc gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca
     5161 aaataaggta tattattgat gatgtttaaa ctacggcccg ccagcttttg ttccctttag
     5221 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5281 tatccgctca caattccaca caacatacga gccgggagca taaagtgtaa agcctggggt
     5341 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     5401 ggaaacctgt cgtgccagct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     5461 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     5521 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     5581 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     5641 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     5701 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     5761 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     5821 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     5881 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     5941 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     6001 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     6061 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     6121 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     6181 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     6241 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     6301 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     6361 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     6421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7021 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     7441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7621 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     7681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     7981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     8401 tcttcaagaa ttctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     8461 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     8521 ctatagggcg aattggagct ccactacgta
//
