LOCUS       sequence-247905-        9300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Histone H2B-superfolder GFP and tdTomato fused by unskippable 
            linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E
FEATURES             Location/Qualifiers
     source          1..9300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(1736..1769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1902..2023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2135..2256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2368..2489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2512..2545)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2567..2944
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     CDS             join(2963..2965,2966..3676)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2963..3676
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2993..3021)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3616..3636
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3764..5194
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(5252..5272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5670..5681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5826..5843)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5832..6056
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6253..6272)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6254..6272)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6279..6296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6279..6295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(6437..6892)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6574..6595)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6786..6805
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6919..7023
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7024..7092,7093..7884)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7242..7261)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8055..8643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8544..8563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8797..8814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8931..8952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8967..8972,8973..8990,8991..8997)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9005..9021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9010..9032
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9029..9045
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9029..9045
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc crggsggggc ggggcggggc grggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg ctagcataac
     1741 ttcgtatagc atacattata cgaagttata gccaccgcgg tggcggccgc tctagaatca
     1801 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     1861 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     1921 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     1981 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaactagaa tcataatcag
     2041 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     2101 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2221 tagttgtggt ttgtccaaac tcatcaatgt atcttaacta gaatcataat cagccatacc
     2281 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2341 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2401 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2461 ggtttgtcca aactcatcaa tgtatcttaa ctagtggatc tcatgcctag gataacttcg
     2521 tatagcatac attatacgaa gttataggcg cgccggtacc gccaccatgc ctgaaccctc
     2581 taagtctgct ccagccccta aaaagggttc taagaaggct atcactaagg cgcagaagaa
     2641 ggatggtaag aagcgtaagc gcagccgcaa ggagagctat tctatctatg tgtacaaggt
     2701 tctgaagcag gtccaccccg acaccggcat ctcatccaag gccatgggga tcatgaactc
     2761 cttcgtcaac gacatcttcg agcgcatcgc gggcgaggct tctcgcctgg ctcactacaa
     2821 taagcgctcg accatcacct ccagggagat tcagacggct gtgcgcctgc tgctgcctgg
     2881 ggagctggct aagcatgctg tgtccgaggg cactaaggca gttaccaagt acactagctc
     2941 taaggatcca ccggtcgcca ccatgagcaa aggagaagaa cttttcactg gagttgtccc
     3001 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg
     3061 tgaaggtgat gctacaaacg gaaaactcac ccttaaattt atttgcacta ctggaaaact
     3121 acctgttccg tggccaacac ttgtcactac tctgacctat ggtgttcaat gcttttcccg
     3181 ttatccggat cacatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
     3241 acaggaacgc actatatctt tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa
     3301 gtttgaaggt gatacccttg ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga
     3361 tggaaacatt cttggacaca aactcgagta caactttaac tcacacaatg tatacatcac
     3421 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acgttgaaga
     3481 tggttccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     3541 ccttttacca gacaaccatt acctgtcgac acaatctgtc ctttcgaaag atcccaacga
     3601 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat
     3661 ggatgagctc tacaaaggag gatctggcgg ccgctctgcc acaaacttca gcctgctgaa
     3721 gcaggccggc gacgccgctg aaaatcctgg ccctggaagc accatggtga gcaagggcga
     3781 ggaggtcatc aaagaattca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     3841 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     3901 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     3961 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     4021 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     4081 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     4141 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     4201 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     4261 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     4321 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     4381 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     4441 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     4501 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     4561 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4621 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     4681 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     4741 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4801 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     4861 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4921 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     4981 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     5041 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     5101 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     5161 gttcctgtac ggcatggacg agctgtacaa gtaaggcgcg ccaacctctg gattacaaaa
     5221 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     5281 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     5341 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     5401 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     5461 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     5521 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     5581 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     5641 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     5701 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5761 ggatctccct ttgggccgcc tccccgcagg atccactaga gcggccgcct agagctcgct
     5821 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5881 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5941 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6001 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6061 tgaggcggaa agaaccagct ggggctcgac tagagcttgc ggaaccctta agcttgtcat
     6121 tcgaatttag acacccacat ggtggctcat gaccatctat ctataaatgg atctgatttt
     6181 catgtctggc agtgtacaga agctaactga agaaaggtgg aagacccaca agagttcaag
     6241 gtacccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     6301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6421 ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     6481 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     6541 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6601 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6661 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6721 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6781 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6841 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     6901 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6961 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7021 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7081 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7141 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7201 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7261 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7321 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7381 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7441 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7501 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7561 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7621 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7681 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7741 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7801 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7861 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7921 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8161 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8821 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8881 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8941 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9001 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     9061 cgcgcaatta agtttaaaca agggcaggat gtgtcaaact gccaatagag aactacttac
     9121 tcttcaggct gaagctgatg gaacaggtaa caaaggcaaa cactaatcat gatcagcaag
     9181 atgaagcaga aagggaacaa ggggatatta aatgtgtata gacacgctag agagatggct
     9241 cagcagttaa gagaactagc tggtctttca gaggtcctga gatcaatacc ggtcatggtc
//
