LOCUS       sequence-247907-        8071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag 
            epitope and NLS and C-terminal fusion of NLS, human p300 HAT core 
            (aa1048-1664), and Flag tag epitope driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, 
            Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, 
            Beisel CL, Barrangou R, Gersbach CA
  TITLE     Targeted transcriptional modulation with type I CRISPR-Cas systems 
            in human cells.
  JOURNAL   Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7.
  PUBMED    31548729
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 
            10.1038/s41587-019-0235-7."
COMMENT     Sequence Label: pcDNA-CasE-p300-Flag
FEATURES             Location/Qualifiers
     source          1..8071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      670..679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             724..744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1354..1401
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1408..3258
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      3022..3031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3265..3288
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3336..3383
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    3434..3658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3704..4132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3791..3810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4001..4022
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4141..4161)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4146..4475
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4326..4461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4388..4407
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4542..5336
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4596..4615)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5206..5225
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5510..5631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5547..5566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5601..5620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5680..5696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5680..5696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5693..5715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5704..5720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5728..5734,5735..5752,5753..5758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5773..5794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5911..5928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6082..6670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6162..6181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6841..7632,7633..7701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7464..7483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7702..7806)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7881..7900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgccg ccaccatggg tgccccagtt ccctatcccg acccactgga gccaaggatg
      721 gcgcctaaga agaagcgcaa ggtgcggggc atgtatctca gtaaagtcat cattgccagg
      781 gcctggagca gggatcttta ccaacttcac cagggattat ggcatttatt tccaaacaga
      841 ccggatgctg ctcgtgattt tctttttcat gttgagaagc gaaacacacc agaaggctgt
      901 catgttttat tgcagtcagc gcaaatgcct gtttcaactg ccgttgcgac agtcattaaa
      961 actaaacagg ttgaatttca acttcaggtt ggtgttccac tctattttcg gcttcgggca
     1021 aatccgatca aaactattct cgacaatcaa aagcgcctgg acagtaaagg gaatattaaa
     1081 cgctgtcggg ttccgttaat aaaagaagca gaacaaatcg cgtggttgca acgtaaattg
     1141 ggcaatgcgg cgcgcgttga agatgtgcat cccatatcgg aacggccaca gtatttttct
     1201 ggtgatggta aaagtggaaa gatccaaacg gtttgctttg aaggtgtgct caccatcaac
     1261 gacgcgccag cgttaataga tcttgtacag caaggtattg ggccagctaa atcgatggga
     1321 tgtggcttgc tatctttggc tccactgcgg ggcaagcgac ctgccgccac aaagaaggct
     1381 ggacaggcta agaagaagaa aggatccatt ttcaaaccag aagaactacg acaggcactg
     1441 atgccaactt tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct
     1501 gtggaccctc agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat
     1561 ctttctacca ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc
     1621 gatgatattt ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta
     1681 tacaaatact gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa
     1741 agccttggat actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac
     1801 ggcaaacagt tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat
     1861 catttctgtg agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatgac
     1921 ccttcccagc ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca
     1981 ctggatcctg aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt
     2041 gtccttcacc atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa
     2101 agtgcacgaa ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt
     2161 ggcacctttc tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca
     2221 ggagaggtca ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc
     2281 atgaaagcaa ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa
     2341 gccctctttg cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt
     2401 caagagtatg gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc
     2461 gatagtgttc atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta
     2521 attggatatt tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt
     2581 ccaccaagtg agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc
     2641 aagcccaagc gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt
     2701 attgtccatg actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca
     2761 aaggaattgc cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag
     2821 gaactggaac aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca
     2881 gatgtgacca agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa
     2941 aataagagca gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac
     3001 gacctctcac agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc
     3061 cgcctcattg ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc
     3121 atcccctgcg atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac
     3181 ctggagttct cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg
     3241 cacacgcaga gccaggacgg atccgattac aaagacgatg acgataagtg agtttaaacc
     3301 gctgatcagc ctcgactgtg agcaatggcc ggggcaagcg acctgccgcc acaaagaagg
     3361 ctggacaggc taagaagaag aaagattaca aagacgatga cgataatgag tttaaaccgc
     3421 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     3481 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3541 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3601 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     3661 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     3721 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3781 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3841 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3901 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3961 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4021 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4081 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     4141 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4201 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4261 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     4321 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4381 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     4441 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     4501 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     4561 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     4621 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     4681 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     4741 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     4801 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     4861 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     4921 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     4981 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5041 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     5101 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5161 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5221 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5281 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5341 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5401 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5461 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     5521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5641 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7801 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     7861 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     7921 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     7981 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8041 cttcgcgatg tacgggccag atatacgcgt t
//
