LOCUS       sequence-24791-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K
  TITLE     Characterization and subcellular targeting of GCaMP-type 
            genetically-encoded calcium indicators.
  JOURNAL   PLoS ONE. 2008  . 3(3):e1796.
  PUBMED    18350138
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(3):e1796."
COMMENT     Sequence Label: pCAGGS-PSD95-GCaMP2
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..483)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     599..618
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgggcngact tagctattca tcggtacctc gagcaccggt tacttcgctg tcatcatttg
       61 tacaaactct tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc
      121 atcaacctct tcatctgtta acttctctcc aaggtttgtc atcacgtggc gaagctctgc
      181 tgcactgatg tagccattgc catccttatc aaacacacgg aacgcttctc taatttcttc
      241 ttcactgtct gtgtctttca tttttcttgc catcattgtc aggaactcag ggaagtcgat
      301 tgtgccatta ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg cttctgtggg
      361 gttctgcccc agagaccgca tcaccgtccc cagctccttg gttgttattg tcccatcccc
      421 gtccttgtca aatagggaga aagcctcttt aaattctgcg atctgctctt cagtcagttg
      481 gtcacgcgtg ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      541 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      601 cttgtagttg ccgtcgtcct tgaagaagnc gggcccncgg ggaatgnacc cttcgggnat
      661 ggcgnacttg aaaaanncgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
      721 gccngnaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      781 agatgaactt cagggtcagc ttgccgtaag tggcatc
//
