LOCUS       sequence-247911-        2372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-psbB 5'UTR processed -54
FEATURES             Location/Qualifiers
     source          1..2372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(578..595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(688..1276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(768..787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1450..2244)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2245..2349)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact caatgcaata aagttacgta gtgtctattt
      421 atctttgata taaggggtat ttccaatgtg agaccggtac cctctgggcc tcatgggcct
      481 tcctttcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc
      541 atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct
      601 cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa
      661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1321 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1381 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1441 tgacagttat tagaaaaatt catccagcag acgataaaac gcaatacgct ggctatccgg
     1501 tgccgcaatg ccatacagca ccagaaaacg atccgcccat tcgccgccca gttcttccgc
     1561 aatatcacgg gtggccagcg caatatcctg ataacgatcc gccacgccca gacggccgca
     1621 atcaataaag ccgctaaaac ggccattttc caccataatg ttcggcaggc acgcatcacc
     1681 atgggtcacc accagatctt cgccatccgg catgctcgct ttcagacgcg caaacagctc
     1741 tgccggtgcc aggccctgat gttcttcatc cagatcatcc tgatccacca ggcccgcttc
     1801 catacgggta cgcgcacgtt caatacgatg tttcgcctga tgatcaaacg gacaggtcgc
     1861 cgggtccagg gtatgcagac gacgcatggc atccgccata atgctcactt tttctgccgg
     1921 cgccagatgg ctagacagca gatcctgacc cggcacttcg cccagcagca gccaatcacg
     1981 gcccgcttcg gtcaccacat ccagcaccgc cgcacacgga acaccggtgg tggccagcca
     2041 gctcagacgc gccgcttcat cctgcagctc gttcagcgca ccgctcagat cggttttcac
     2101 aaacagcacc ggacgaccct gcgcgctcag acgaaacacc gccgcatcag agcagccaat
     2161 ggtctgctgc gcccaatcat agccaaacag acgttccacc cacgctgccg ggctacccgc
     2221 atgcaggcca tcctgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca
     2281 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2341 ggttccgcgc acatttcccc gaaaagtgcc ac
//
