LOCUS       sequence-247920-        2451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-ndhD 5'UTR
FEATURES             Location/Qualifiers
     source          1..2451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(657..674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(767..1355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(847..866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1529..2323)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2324..2428)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catacttacg ttcgagaaaa ctctacttga
      421 atccatttaa ttttttttac cgacaaacct gtgctcgaaa atcacaatat tttgagcacg
      481 ggtttttatg gtccaagtgt atcttgtctt tactaatgtg agaccggtac ctggagcaca
      541 agactggcct catgggcctt cctttcactg cccgctttcc agtcgggaaa cctgtcgtgc
      601 cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg
      661 ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag
      721 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      781 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      841 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      901 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      961 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1021 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1081 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1141 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1201 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1261 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1321 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1381 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1441 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1501 tatatgagta aacttggtct gacagttatt agaaaaattc atccagcaga cgataaaacg
     1561 caatacgctg gctatccggt gccgcaatgc catacagcac cagaaaacga tccgcccatt
     1621 cgccgcccag ttcttccgca atatcacggg tggccagcgc aatatcctga taacgatccg
     1681 ccacgcccag acggccgcaa tcaataaagc cgctaaaacg gccattttcc accataatgt
     1741 tcggcaggca cgcatcacca tgggtcacca ccagatcttc gccatccggc atgctcgctt
     1801 tcagacgcgc aaacagctct gccggtgcca ggccctgatg ttcttcatcc agatcatcct
     1861 gatccaccag gcccgcttcc atacgggtac gcgcacgttc aatacgatgt ttcgcctgat
     1921 gatcaaacgg acaggtcgcc gggtccaggg tatgcagacg acgcatggca tccgccataa
     1981 tgctcacttt ttctgccggc gccagatggc tagacagcag atcctgaccc ggcacttcgc
     2041 ccagcagcag ccaatcacgg cccgcttcgg tcaccacatc cagcaccgcc gcacacggaa
     2101 caccggtggt ggccagccag ctcagacgcg ccgcttcatc ctgcagctcg ttcagcgcac
     2161 cgctcagatc ggttttcaca aacagcaccg gacgaccctg cgcgctcaga cgaaacaccg
     2221 ccgcatcaga gcagccaatg gtctgctgcg cccaatcata gccaaacaga cgttccaccc
     2281 acgctgccgg gctacccgca tgcaggccat cctgttcaat catactcttc ctttttcaat
     2341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
