LOCUS       sequence-247921-        2360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rps2 5'UTR
FEATURES             Location/Qualifiers
     source          1..2360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(566..583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(676..1264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(756..775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1438..2232)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2233..2337)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact ttttgaaaaa gaataaaaaa aaaagggggg
      421 gtgtggagag aaaatgtgag accggtaccc tctgggcctc atgggccttc ctttcactgc
      481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc
      541 ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg
      601 taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttatta
     1441 gaaaaattca tccagcagac gataaaacgc aatacgctgg ctatccggtg ccgcaatgcc
     1501 atacagcacc agaaaacgat ccgcccattc gccgcccagt tcttccgcaa tatcacgggt
     1561 ggccagcgca atatcctgat aacgatccgc cacgcccaga cggccgcaat caataaagcc
     1621 gctaaaacgg ccattttcca ccataatgtt cggcaggcac gcatcaccat gggtcaccac
     1681 cagatcttcg ccatccggca tgctcgcttt cagacgcgca aacagctctg ccggtgccag
     1741 gccctgatgt tcttcatcca gatcatcctg atccaccagg cccgcttcca tacgggtacg
     1801 cgcacgttca atacgatgtt tcgcctgatg atcaaacgga caggtcgccg ggtccagggt
     1861 atgcagacga cgcatggcat ccgccataat gctcactttt tctgccggcg ccagatggct
     1921 agacagcaga tcctgacccg gcacttcgcc cagcagcagc caatcacggc ccgcttcggt
     1981 caccacatcc agcaccgccg cacacggaac accggtggtg gccagccagc tcagacgcgc
     2041 cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa acagcaccgg
     2101 acgaccctgc gcgctcagac gaaacaccgc cgcatcagag cagccaatgg tctgctgcgc
     2161 ccaatcatag ccaaacagac gttccaccca cgctgccggg ctacccgcat gcaggccatc
     2221 ctgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2341 atttccccga aaagtgccac
//
