LOCUS       sequence-247925-        2350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rps2 5'UTR mAAGA (-20 to -10 deletion)
FEATURES             Location/Qualifiers
     source          1..2350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(556..573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(666..1254)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(746..765)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1428..2222)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2223..2327)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact ttttgaaaaa gaataaaaaa aagtaagaag
      421 aaaatgtgag accggtaccc tctgggcctc atgggccttc ctttcactgc ccgctttcca
      481 gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg
      541 ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg
      601 ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      661 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      721 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      781 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      841 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      901 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      961 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1021 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1081 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1141 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1201 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1261 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1321 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1381 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttatta gaaaaattca
     1441 tccagcagac gataaaacgc aatacgctgg ctatccggtg ccgcaatgcc atacagcacc
     1501 agaaaacgat ccgcccattc gccgcccagt tcttccgcaa tatcacgggt ggccagcgca
     1561 atatcctgat aacgatccgc cacgcccaga cggccgcaat caataaagcc gctaaaacgg
     1621 ccattttcca ccataatgtt cggcaggcac gcatcaccat gggtcaccac cagatcttcg
     1681 ccatccggca tgctcgcttt cagacgcgca aacagctctg ccggtgccag gccctgatgt
     1741 tcttcatcca gatcatcctg atccaccagg cccgcttcca tacgggtacg cgcacgttca
     1801 atacgatgtt tcgcctgatg atcaaacgga caggtcgccg ggtccagggt atgcagacga
     1861 cgcatggcat ccgccataat gctcactttt tctgccggcg ccagatggct agacagcaga
     1921 tcctgacccg gcacttcgcc cagcagcagc caatcacggc ccgcttcggt caccacatcc
     1981 agcaccgccg cacacggaac accggtggtg gccagccagc tcagacgcgc cgcttcatcc
     2041 tgcagctcgt tcagcgcacc gctcagatcg gttttcacaa acagcaccgg acgaccctgc
     2101 gcgctcagac gaaacaccgc cgcatcagag cagccaatgg tctgctgcgc ccaatcatag
     2161 ccaaacagac gttccaccca cgctgccggg ctacccgcat gcaggccatc ctgttcaatc
     2221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2341 aaagtgccac
//
