LOCUS       sequence-24793-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K
  TITLE     Characterization and subcellular targeting of GCaMP-type 
            genetically-encoded calcium indicators.
  JOURNAL   PLoS ONE. 2008  . 3(3):e1796.
  PUBMED    18350138
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(3):e1796."
COMMENT     Sequence Label: pCAGGS-hCD4-GCaMP2
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..485)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     601..620
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggcggcnga cttagctatt catcggtacc tcgagcaccg gttacttcgc tgtcatcatt
       61 tgtacaaact cttcgtagtt tacctgacca tccccatcga tgtctgcttc cctgatcatt
      121 tcatcaacct cttcatctgt taacttctct ccaaggtttg tcatcacgtg gcgaagctct
      181 gctgcactga tgtagccatt gccatcctta tcaaacacac ggaacgcttc tctaatttct
      241 tcttcactgt ctgtgtcttt catttttctt gccatcattg tcaggaactc agggaagtcg
      301 attgtgccat taccgtcggc atctacttca ttgatcatgt cctgcagctc tgcttctgtg
      361 gggttctgcc ccagagaccg catcaccgtc cccagctcct tggttgttat tgtcccatcc
      421 ccgtccttgt caaataggga gaaagcctct ttaaattctg cgatctgctc ttcagtcagt
      481 tggtcacgcg tgttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      541 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      601 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcngn gg
//
