LOCUS       sequence-247932-       10263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 at nuclear pore.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: V5-APEX2-SENP2
FEATURES             Location/Qualifiers
     source          1..10263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             682..1428
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     misc_feature    3270..3858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3323..3343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3741..3752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3930..4163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4241..4362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4278..4297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4332..4351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4402..4537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4557..4576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4558..4576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4586..4603)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4586..4602)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4595..4617)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4744..5199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4831..4850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5041..5062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5225..5329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5330..5398,5399..6190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5548..5567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6361..6949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6850..6869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7103..7120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7237..7258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7273..7278,7279..7296,7297..7303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7311..7327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7316..7338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7335..7351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7335..7351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7370..7390
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7372..7390
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7418..7644
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7645..7825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7872..7997
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8490..8723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8908..8952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9127..9627
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9546..9564
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9639..10034
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10094..10211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg gcaagcccat ccccaacccc
      661 ctgctgggcc tggacagcac cggaaagtct tacccaactg tgagtgctga ttaccaggac
      721 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct
      781 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccggt
      841 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt
      901 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc
      961 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca
     1021 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcctgatccc
     1081 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa
     1141 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga
     1201 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg
     1261 ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct tttgtctgac
     1321 cctgtattcc gccctctcgt tgataaatat gcagcggacg aagatgcctt ctttgctgat
     1381 tacgctgagg ctcaccaaaa gctttccgag cttgggtttg ctgatgcctc cggccggact
     1441 cagatctcga gctcaagctt cgaattcatg tacagatggc tggttaggat tctcggcacc
     1501 attttccgtt tctgcgaccg gtcggtgccc cctgcccggg ccctcctgaa gaggcggcgc
     1561 tcagacagca ctctgttttc tacagtggac actgatgaaa taccagccaa aagaccaaga
     1621 ttagattgct ttattcacca agtgaaaaac agtctctaca atgctgccag cttatttgga
     1681 ttcccattcc agctgaccac aaagcccatg gtaacttctg cttgtaatgg aacacggaat
     1741 gtggcccctt caggagaggt attttcgaac tcttcatctt gtgaactgac aggttctgga
     1801 tcctggaaca acatgctgaa actgggtaat aaatctccta atggaataag tgactatcca
     1861 aagatcagag tgacagttac ccgagatcag ccacgcagag tcctgccttc ctttggtttt
     1921 actttgaact cagaaggctg taatagaaga ccaggtggcc gtcgccatag caaaggtaat
     1981 ccagagagtt ctttaatgtg gaaacctcag gaacaggctg taacagagat gatttctgaa
     2041 gagagtggca agggtctgag gcgtccccat tgtactgtgg aggagggtgt tcaaaaagag
     2101 gaaagagaga agtaccgaaa gttattggaa cgacttaaag aaagtggtca tggaaactct
     2161 gtctgtcctg taacttcaaa ttatcacagt tctcaaagaa gtcagatgga cacattaaag
     2221 accaaaggct ggggggaaga gcaaaatcac ggagtcaaaa caactcagtt tgttccaaaa
     2281 caatatagac ttgttgaaac aaggggacct ctatgttcat tgagaagtga aaagaggtgt
     2341 tcaaagggga aaattactga tacagagacg atggtcggaa tcagatttga aaatgaaagt
     2401 aggaggggat accaactgga gcctgaccta tcagaagaag tgtcggcccg actccgcctg
     2461 ggcagtggaa gcaatggctt actcaggagg aaagtgtcaa taattgagac aaaggaaaag
     2521 aattgctcag gcaaagagag ggacagaaga acggacgatc tccttgaact tacagaggac
     2581 atggaaaagg aaatcagtaa tgccctaggc catggcccac aggatgaaat cctaagtagt
     2641 gctttcaaat tgcgaattac tcgaggagat attcagacat taaagaacta tcactggctc
     2701 aatgatgaag tcattaattt ttacatgaat cttctggtgg aaagaaataa aaagcaaggc
     2761 tatccagcac ttcatgtatt cagtactttc ttctatccta aattaaagtc tgggggttac
     2821 caagcagtga aacgatggac caaaggggta aatctctttg aacaagaaat tattctggtg
     2881 cctattcatc ggaaggtaca ttggagcctg gtggtgattg acctaagaaa aaagtgtctt
     2941 aaatatctgg attctatggg acaaaagggc cacaggatct gtgagattct ccttcagtat
     3001 ttacaggatg aaagtaagac caaaagaaat agtgatctga atcttttaga gtggacccat
     3061 cacagcatga aaccacacga gattcctcaa cagctgaatg ggagtgattg tggaatgttt
     3121 acttgtaaat atgcagatta tatttctagg gacaaaccta tcacatttac tcagcaccag
     3181 atgcctctct tccggaagaa gatggtgtgg gaaatccttc atcagcagtt gctgtgagct
     3241 agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     3301 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3361 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3421 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3481 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3541 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3661 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3721 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3781 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3841 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     3901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4141 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     4201 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     4261 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4321 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     4381 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4441 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4501 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     4561 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4621 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4681 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4741 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5161 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     5221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5401 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5461 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5521 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5581 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5641 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5701 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5761 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5821 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5881 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5941 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6001 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6061 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6121 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6181 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7021 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7081 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7141 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7201 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7261 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7321 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7381 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7441 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7501 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7561 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7621 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     7681 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7741 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7801 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7861 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7921 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7981 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     8041 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     8101 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8161 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8221 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8281 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8341 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8401 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8461 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8521 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8581 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8641 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8701 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8761 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     8821 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     8881 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     8941 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     9001 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     9061 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9121 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     9181 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     9241 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     9301 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     9361 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     9421 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     9481 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     9541 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     9601 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     9661 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     9721 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     9781 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     9841 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     9901 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     9961 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
    10021 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag
    10081 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10141 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10201 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10261 ggg
//
