LOCUS       sequence-247933-        2392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL)
FEATURES             Location/Qualifiers
     source          1..2392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(598..615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(708..1296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(788..807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1470..2264)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2265..2369)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc aacgagctcg gtctcatact atgtatttgg caaatcaaat accatgctag
      421 tgtgcttggg agtccctgat gattaaataa accaagattt taccaatgtg agaccggtac
      481 cctctgggcc tcatgggcct tcctttcact gcccgctttc cagtcgggaa acctgtcgtg
      541 ccagctgcat taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc
      601 gctcactgac tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa
      661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1261 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1381 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1441 atatatgagt aaacttggtc tgacagttat tagaaaaatt catccagcag acgataaaac
     1501 gcaatacgct ggctatccgg tgccgcaatg ccatacagca ccagaaaacg atccgcccat
     1561 tcgccgccca gttcttccgc aatatcacgg gtggccagcg caatatcctg ataacgatcc
     1621 gccacgccca gacggccgca atcaataaag ccgctaaaac ggccattttc caccataatg
     1681 ttcggcaggc acgcatcacc atgggtcacc accagatctt cgccatccgg catgctcgct
     1741 ttcagacgcg caaacagctc tgccggtgcc aggccctgat gttcttcatc cagatcatcc
     1801 tgatccacca ggcccgcttc catacgggta cgcgcacgtt caatacgatg tttcgcctga
     1861 tgatcaaacg gacaggtcgc cgggtccagg gtatgcagac gacgcatggc atccgccata
     1921 atgctcactt tttctgccgg cgccagatgg ctagacagca gatcctgacc cggcacttcg
     1981 cccagcagca gccaatcacg gcccgcttcg gtcaccacat ccagcaccgc cgcacacgga
     2041 acaccggtgg tggccagcca gctcagacgc gccgcttcat cctgcagctc gttcagcgca
     2101 ccgctcagat cggttttcac aaacagcacc ggacgaccct gcgcgctcag acgaaacacc
     2161 gccgcatcag agcagccaat ggtctgctgc gcccaatcat agccaaacag acgttccacc
     2221 cacgctgccg ggctacccgc atgcaggcca tcctgttcaa tcatactctt cctttttcaa
     2281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2341 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
