LOCUS       sequence-247934-        2441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-psbA-rbcL chimeric 5'UTR (R'SLWT)
FEATURES             Location/Qualifiers
     source          1..2441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(647..664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(757..1345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(837..856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1519..2313)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2314..2418)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catactatgt atttggcaaa tcaaatacca
      421 tggtctaata atcaaacatt ctgattagtt gataatctag tgtgcttggg agtccctgat
      481 gattaaataa accaagattt taccaatgtg agaccggtac ctggagcaca agactggcct
      541 catgggcctt cctttcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      601 aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg ctcactgact
      661 cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa
      721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1501 aacttggtct gacagttatt agaaaaattc atccagcaga cgataaaacg caatacgctg
     1561 gctatccggt gccgcaatgc catacagcac cagaaaacga tccgcccatt cgccgcccag
     1621 ttcttccgca atatcacggg tggccagcgc aatatcctga taacgatccg ccacgcccag
     1681 acggccgcaa tcaataaagc cgctaaaacg gccattttcc accataatgt tcggcaggca
     1741 cgcatcacca tgggtcacca ccagatcttc gccatccggc atgctcgctt tcagacgcgc
     1801 aaacagctct gccggtgcca ggccctgatg ttcttcatcc agatcatcct gatccaccag
     1861 gcccgcttcc atacgggtac gcgcacgttc aatacgatgt ttcgcctgat gatcaaacgg
     1921 acaggtcgcc gggtccaggg tatgcagacg acgcatggca tccgccataa tgctcacttt
     1981 ttctgccggc gccagatggc tagacagcag atcctgaccc ggcacttcgc ccagcagcag
     2041 ccaatcacgg cccgcttcgg tcaccacatc cagcaccgcc gcacacggaa caccggtggt
     2101 ggccagccag ctcagacgcg ccgcttcatc ctgcagctcg ttcagcgcac cgctcagatc
     2161 ggttttcaca aacagcaccg gacgaccctg cgcgctcaga cgaaacaccg ccgcatcaga
     2221 gcagccaatg gtctgctgcg cccaatcata gccaaacaga cgttccaccc acgctgccgg
     2281 gctacccgca tgcaggccat cctgttcaat catactcttc ctttttcaat attattgaag
     2341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2401 acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
