LOCUS       sequence-247935-       10497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 at nuclear lamina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 10497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: V5-APEX2-LMNA
FEATURES             Location/Qualifiers
     source          1..10497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             682..1428
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1447..1476
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3504..4092
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3557..3577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3975..3986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4164..4397
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4475..4596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4512..4531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4566..4585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4636..4771
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4791..4810)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4792..4810)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4820..4837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4820..4836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4829..4851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4978..5433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5065..5084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5275..5296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5459..5563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5564..5632,5633..6424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5782..5801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6595..7183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7084..7103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7337..7354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7471..7492
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7507..7512,7513..7530,7531..7537)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7545..7561
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7550..7572
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7569..7585
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7569..7585
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7604..7624
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7606..7624
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7652..7878
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7879..8059
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8106..8231
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8724..8957
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9142..9186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9361..9861
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9780..9798
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9873..10268
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10328..10445
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg gcaagcccat ccccaacccc
      661 ctgctgggcc tggacagcac cggaaagtct tacccaactg tgagtgctga ttaccaggac
      721 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct
      781 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccggt
      841 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt
      901 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc
      961 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca
     1021 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcctgatccc
     1081 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa
     1141 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga
     1201 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg
     1261 ttgagtggtg agaaggaagg tctccttcag ctaccttctg acaaggctct tttgtctgac
     1321 cctgtattcc gccctctcgt tgataaatat gcagcggacg aagatgcctt ctttgctgat
     1381 tacgctgagg ctcaccaaaa gctttccgag cttgggtttg ctgatgcctc cggactcaga
     1441 tctatggagc aaaagctcat ttctgaagag gacttgaatg agaccccgtc ccagcggcgc
     1501 gccacccgca gcggggcgca ggccagctcc actccgctgt cgcccacccg catcacccgg
     1561 ctgcaggaga aggaggacct gcaggagctc aatgatcgct tggcggtcta catcgaccgt
     1621 gtgcgctcgc tggaaacgga gaacgcaggg ctgcgccttc gcatcaccga gtctgaagag
     1681 gtggtcagcc gcgaggtgtc cggcatcaag gccgcctacg aggccgagct cggggatgcc
     1741 cgcaagaccc ttgactcagt agccaaggag cgcgcccgcc tgcagctgga gctgagcaaa
     1801 gtgcgtgagg agtttaagga gctgaaagcg cgcaatacca agaaggaggg tgacctgata
     1861 gctgctcagg ctcggctgaa ggacctggag gctctgctga actccaagga ggccgcactg
     1921 agcactgctc tcagtgagaa gcgcacgctg gagggcgagc tgcatgatct gcggggccag
     1981 gtggccaagc ttgaggcagc cctaggtgag gccaagaagc aacttcagga tgagatgctg
     2041 cggcgggtgg atgctgagaa caggctgcag accatgaagg aggaactgga cttccagaag
     2101 aacatctaca gtgaggagct gcgtgagacc aagcgccgtc atgagacccg actggtggag
     2161 attgacaatg ggaagcagcg tgagtttgag agccggctgg cggatgcgct gcaggaactg
     2221 cgggcccagc atgaggacca ggtggagcag tataagaagg agctggagaa gacttattct
     2281 gccaagctgg acaatgccag gcagtctgct gagaggaaca gcaacctggt gggggctgcc
     2341 cacgaggagc tgcagcagtc gcgcatccgc atcgacagcc tctctgccca gctcagccag
     2401 ctccagaagc agctggcagc caaggaggcg aagcttcgag acctggagga ctcactggcc
     2461 cgtgagcggg acaccagccg gcggctgctg gcggaaaagg agcgggagat ggccgagatg
     2521 cgggcaagga tgcagcagca gctggacgag taccaggagc ttctggacat caagctggcc
     2581 ctggacatgg agatccacgc ctaccgcaag ctcttggagg gcgaggagga gaggctacgc
     2641 ctgtccccca gccctacctc gcagcgcagc cgtggccgtg cttcctctca ctcatcccag
     2701 acacagggtg ggggcagcgt caccaaaaag cgcaaactgg agtccactga gagccgcagc
     2761 agcttctcac agcacgcacg cactagcggg cgcgtggccg tggaggaggt ggatgaggag
     2821 ggcaagtttg tccggctgcg caacaagtcc aatgaggacc agtccatggg caattggcag
     2881 atcaagcgcc agaatggaga tgatcccttg ctgacttacc ggttcccacc aaagttcacc
     2941 ctgaaggctg ggcaggtggt gacgatctgg gctgcaggag ctggggccac ccacagcccc
     3001 cctaccgacc tggtgtggaa ggcacagaac acctggggct gcgggaacag cctgcgtacg
     3061 gctctcatca actccactgg ggaagaagtg gccatgcgca agctggtgcg ctcagtgact
     3121 gtggttgagg acgacgagga tgaggatgga gatgacctgc tccatcacca ccacggctcc
     3181 cactgcagca gctcggggga ccccgctgag tacaacctgc gctcgcgcac cgtgctgtgc
     3241 gggacctgcg ggcagcctgc cgacaaggca tctgccagcg gctcaggagc ccaggtgggc
     3301 ggacccatct cctctggctc ttctgcctcc agtgtcacgg tcactcgcag ctaccgcagt
     3361 gtggggggca gtgggggtgg cagcttcggg gacaatctgg tcacccgctc ctacctcctg
     3421 ggcaactcca gcccccgaac ccagagcccc cagaactgca gcatcatgta agctagcgct
     3481 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3541 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3601 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3661 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3721 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3781 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3841 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3901 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3961 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4021 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4081 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     4141 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     4201 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4261 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4321 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4381 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     4441 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     4501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     4621 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4681 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     4741 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     4801 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4861 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4921 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4981 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5041 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5101 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5161 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5221 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5281 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5341 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5401 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     5461 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     5521 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     5581 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     5641 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     5701 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     5761 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5821 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5881 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5941 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6001 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6061 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6121 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6181 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6241 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6301 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6361 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     6421 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     6481 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     6541 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6601 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6661 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6721 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6781 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6841 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6901 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6961 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7021 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7081 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7141 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7201 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7261 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7321 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     7381 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7441 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     7501 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     7561 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     7621 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     7681 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     7741 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     7801 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7861 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7921 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7981 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8041 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8101 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8161 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     8221 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     8281 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     8341 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     8401 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     8461 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     8521 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     8581 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     8641 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     8701 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8761 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8821 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8881 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8941 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     9001 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     9061 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     9121 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     9181 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     9241 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     9301 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     9361 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     9421 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     9481 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     9541 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     9601 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     9661 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     9721 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     9781 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     9841 atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct caagaagaat
     9901 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     9961 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
    10021 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
    10081 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
    10141 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
    10201 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
    10261 gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg gcagtattca
    10321 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10381 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10441 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggggg
//
