LOCUS       sequence-247936-        2455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rps14 5'UTR
FEATURES             Location/Qualifiers
     source          1..2455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(661..678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(771..1359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(851..870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1533..2327)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2328..2432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catacttatt tttaatgtgt gtatctgcga
      421 taatctcatt tctttcgacg gagagggggt ccaccttctt ctatttctac atctaggatt
      481 cgacttgtat catggatact aataggaatt caaccattaa tgtgagaccg gtacctggag
      541 cacaagactg gcctcatggg ccttcctttc actgcccgct ttccagtcgg gaaacctgtc
      601 gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc
      661 ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1501 agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag cagacgataa
     1561 aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa acgatccgcc
     1621 cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc ctgataacga
     1681 tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt ttccaccata
     1741 atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc cggcatgctc
     1801 gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc atccagatca
     1861 tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg atgtttcgcc
     1921 tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat ggcatccgcc
     1981 ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg acccggcact
     2041 tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac cgccgcacac
     2101 ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag ctcgttcagc
     2161 gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct cagacgaaac
     2221 accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa cagacgttcc
     2281 acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact cttccttttt
     2341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
