LOCUS       sequence-247937-        2515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoChlo kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, 
            Lenaghan S
  TITLE     MoChlo: A versatile modular cloning toolbox for chloroplast 
            biotechnology.
  JOURNAL   Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 
            10.1104/pp.18.01220.
  PUBMED    30679266
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220."
COMMENT     Sequence Label: pMK-rbcL 5'UTR (full)
FEATURES             Location/Qualifiers
     source          1..2515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(721..738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(831..1419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(911..930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1593..2387)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2388..2492)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccacgtg tcttgtccag agctcggtct catactatgt atttggcaaa tcaaatacca
      421 tggtctaata atcaaacatt ctgattagtt gataatatta gtattagttg gaaattttgt
      481 gaaagattcc tgtgaaaagt ttcattaaca cggaattcgt gtcgagtaga ccttgttgtt
      541 gtgagaattc ttaattcatg agttgtaggg agggatttaa tgtgagaccg gtacctggag
      601 cacaagactg gcctcatggg ccttcctttc actgcccgct ttccagtcgg gaaacctgtc
      661 gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc tctccgcttc
      721 ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca
      781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1261 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1501 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1561 agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag cagacgataa
     1621 aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa acgatccgcc
     1681 cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc ctgataacga
     1741 tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt ttccaccata
     1801 atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc cggcatgctc
     1861 gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc atccagatca
     1921 tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg atgtttcgcc
     1981 tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat ggcatccgcc
     2041 ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg acccggcact
     2101 tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac cgccgcacac
     2161 ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag ctcgttcagc
     2221 gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct cagacgaaac
     2281 accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa cagacgttcc
     2341 acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact cttccttttt
     2401 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2461 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
