LOCUS       sequence-247942-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of APEX2 in the nucleolus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Fazal FM, Han S, Parker KR, Kaewsapsak P, Xu J, Boettiger AN, Chang 
            HY, Ting AY
  TITLE     Atlas of Subcellular RNA Localization Revealed by APEX-Seq.
  JOURNAL   Cell. 2019 Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027.
  PUBMED    31230715
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 14. pii: S0092-8674(19)30555-0. doi: 
            10.1016/j.cell.2019.05.027."
COMMENT     Sequence Label: GFP-APEX2-NIK3x
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(625..627,628..630,631..1341)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(625..627,628..630,631..1341)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(670..691)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(931..950)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1278..1299
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1363..2109
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    complement(2240..2264)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2266..2307
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2349..2937
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2402..2422)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2820..2831)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3009..3242
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3320..3441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3357..3376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3411..3430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3481..3616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3636..3655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3637..3655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3665..3682)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3665..3681)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3674..3696)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3823..4278
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3910..3929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4120..4141
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4304..4408
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4409..4477,4478..5269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4627..4646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5440..6028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5929..5948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6182..6199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6316..6337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6352..6357,6358..6375,6376..6382)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6390..6406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6395..6417
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6414..6430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6414..6430
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6449..6469
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6451..6469
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6497..6723
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6724..6904
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6951..7076
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7569..7802
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7987..8031
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8206..8706
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8625..8643
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8718..9113
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9173..9290
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt caccatggtg agcaagggcg aggagctgtt caccggggtg
      661 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      721 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      781 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      841 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      901 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      961 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1021 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1081 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1141 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1201 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1261 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1321 ggcatggacg agctgtacaa gtccggactc agatctcgag ctggaaagtc ttacccaact
     1381 gtgagtgctg attaccagga cgccgttgag aaggcgaaga agaagctcag aggcttcatc
     1441 gctgagaaga gatgcgctcc tctaatgctc cgtttggcat tccactctgc tggaaccttt
     1501 gacaagggca cgaagaccgg tggacccttc ggaaccatca agcaccctgc cgaactggct
     1561 cacagcgcta acaacggtct tgacatcgct gttaggcttt tggagccact caaggcggag
     1621 ttccctattt tgagctacgc cgatttctac cagttggctg gcgttgttgc cgttgaggtc
     1681 acgggtggac ctaaggttcc attccaccct ggaagagagg acaagcctga gccaccacca
     1741 gagggtcgct tgcctgatcc cactaagggt tctgaccatt tgagagatgt gtttggcaaa
     1801 gctatggggc ttactgacca agatatcgtt gctctatctg ggggtcacac tattggagct
     1861 gcacacaagg agcgttctgg atttgagggt ccctggacct ctaatcctct tattttcgac
     1921 aactcatact tcacggagtt gttgagtggt gagaaggaag gtctccttca gctaccttct
     1981 gacaaggctc ttttgtctga ccctgtattc cgccctctcg ttgataaata tgcagcggac
     2041 gaagatgcct tctttgctga ttacgctgag gctcaccaaa agctttccga gcttgggttt
     2101 gctgatgcct cgaattcaag aatgagaaag aagcgaaaaa agaagcttcg aatcctgcgc
     2161 atgagaaaga agcgcaagaa gaagctccgc atcctcagga tgcgcaagaa acgcaagaag
     2221 aagctgcgca tcctgtgaga cccagctttc ttgtacaaag tggttggtaa gcctatccct
     2281 aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
     2341 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2401 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2461 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2521 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2581 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2641 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2701 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2761 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2821 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2881 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     2941 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3001 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     3061 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3121 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3181 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3241 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     3301 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     3361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3421 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     3481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3541 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3601 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     3661 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     3721 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     3781 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     3841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3901 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3961 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4021 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4081 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4141 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4201 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4261 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4321 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4381 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4441 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4501 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4561 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4621 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4681 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4741 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4801 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4861 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4921 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4981 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5041 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5101 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5161 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5221 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5281 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5341 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5401 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5461 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5521 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5581 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5641 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5701 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5761 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5821 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5881 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5941 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6001 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6061 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6121 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6181 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6241 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6301 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6361 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6421 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6481 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     6541 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     6601 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     6661 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     6721 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6781 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6841 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6901 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     6961 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     7021 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     7081 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     7141 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     7201 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     7261 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     7321 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     7381 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     7441 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     7501 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     7561 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     7621 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     7681 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7741 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7801 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7861 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7921 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7981 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     8041 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     8101 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     8161 tcaccattat cgtttcagac ccacctccca accccgaggg gacccttgcg ccttttccaa
     8221 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     8281 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     8341 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     8401 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     8461 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     8521 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     8581 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     8641 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     8701 ccccaggggg taccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     8761 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     8821 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     8881 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     8941 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     9001 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     9061 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggcctgcagc
     9121 tgcagtagta agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     9181 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9241 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9301 acagggacag cagagatcca ctttggcgcc ggctcgaggg gg
//
