LOCUS       sequence-247956-        8312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM 
            N-terminus, unskippable linker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..8312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1734..2444
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             2466..2519
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2535..2537,2538..3248)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2535..3248
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2565..2593)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3188..3208
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4377..5312
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4425..5318
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5330..5349)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5395..5450
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5449..5468)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5811..5827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5811..5827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5824..5846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5835..5851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5859..5865,5866..5883,5884..5889))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5904..5925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5984..6179
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6030..6165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6185..6319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6222..6241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6276..6295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6387..6404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6558..7146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6638..6657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7317..8108,8109..8177))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7940..7959
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8178..8282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc cgggcggggc ggggcggggc ggggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gccatggtgt
     1741 ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     1801 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1861 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1921 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     1981 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     2041 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     2101 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     2161 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     2221 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata
     2281 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     2341 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     2401 ccagatactg cgacctccct agcaaactgg ggcacaagct taatggcggc tccggcggcc
     2461 gctccgaggg ccgcggcagc ctgctgacat gcggcgacgt ggaagagaac cctggccctg
     2521 ctcctggcag cacaatgagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
     2581 ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag ggtgaaggtg
     2641 atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2701 cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc cgttatccgg
     2761 atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
     2821 gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc aagtttgaag
     2881 gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa gatggaaaca
     2941 ttcttggaca caaactcgag tacaacttta actcacacaa tgtatacatc acggcagaca
     3001 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa gatggttccg
     3061 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     3121 cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac gaaaagcgtg
     3181 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
     3241 tctacaaagg aggatctggc ggccgctctg ccacaaactt cagcctgctg aagcaggccg
     3301 gcgacgccgc tgaaaatcct ggccctgctc ctggaagcac caacctgctg actgtgcacc
     3361 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg
     3421 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt
     3481 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg
     3541 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc
     3601 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg
     3661 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg
     3721 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga
     3781 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     3841 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     3901 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     3961 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     4021 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     4081 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     4141 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     4201 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa
     4261 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     4321 actctgagac tggggccatg gtgaggctgc tagaggatgg ggacctcgag ccatctgctg
     4381 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
     4441 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     4501 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     4561 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     4621 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     4681 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     4741 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     4801 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     4861 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     4921 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     4981 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     5041 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     5101 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     5161 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     5221 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     5281 tcacggggga ggcagagggt ttccctgcca cagcttgagg cgcgccggat ctttttccct
     5341 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     5401 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     5461 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     5521 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     5581 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     5641 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     5701 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     5761 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     5821 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5881 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5941 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     6001 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6061 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6121 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6181 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6241 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6301 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8281 cgcacatttc cccgaaaagt gccacctggg tc
//
